BMKMANU S1000 Spatial Transcriptome
BMKMANU S1000 Spatial Transcriptome Technical Scheme
Pono o BMKMANU S1000
1) Hoʻonā Sub-cellular: Loaʻa i kēlā me kēia wahi hopu he> 2 miliona spatial Barcoded Spots me ke anawaena o 2.5 µm a me kahi ākea o 5 µm ma waena o nā kikowaena kiko, e hiki ai i ka nānā ʻana i ka transcriptome spatial me ka hoʻonā sub-cellular (5 µm).
2) Ka nānā 'ana i ka ho'onā 'ana i nā pae he nui: 'O ka nānā 'ana i nā pae he nui ma'alahi mai ka 100 μm a hiki i ka 5 μm no ka ho'oholo 'ana i nā hi'ohi'ona 'oko'a ma ka ho'onā kūpono.
3) Ka hoʻopili ʻana i ka transcriptome profiling: Hiki ke kālailai ʻia nā kope i hopu ʻia mai ka paheʻe ʻiʻo holoʻokoʻa, me ka ʻole o ka kaupalena ʻana i ka helu o nā genes a me ka wahi i kuhikuhi ʻia.
Nā kikoʻī lawelawe
| Hale Waihona Puke | Hoʻolālā kaʻina | Hoʻopuka ʻia ka ʻikepili |
| S1000 cDNA waihona | BMKMANU S1000-Illumina PE150 | 60Gb / hāpana |
Nā Koina Laʻana
| Laʻana | Helu | Nui | RNA maikaʻi |
| ʻOCT i hoʻokomo ʻia i ka ʻiʻo poloka | 2-3 poloka/ hāpana | Ma kahi.6.8x6.8x6.8 mm3 | RIN≥7 |
No nā kikoʻī hou aku e pili ana i ke alakaʻi hoʻomākaukau laʻana a me ke kahe hana lawelawe, e ʻoluʻolu e kamaʻilio me aloea BMKGENE
Holo Hana Hana
Hoʻolālā hoʻokolohua
Hāʻawi laʻana
Hoʻāʻo mua
Hoʻopaʻa paʻi kikoʻī
Kūkulu hale waihona puke
Ke kaʻina ʻana
ʻIkepili ʻikepili
Nā lawelawe ma hope o ke kūʻai aku
ʻIke ʻia nā ʻikepili i hana ʻia e BMKMANU S1000 me ka hoʻohana ʻana i ka polokalamu "BSTMatrix", i hoʻolālā kūʻokoʻa ʻia e BMKGENE, me:
1) Hoʻokumu ʻana i ka matrix hōʻike Gene
2) HE hoʻoponopono kiʻi
3) Hoʻohālikelike me nā polokalamu ʻaoʻao ʻekolu no ka nānā ʻana
4) Kōkua ʻo "BSTViewer" pūnaewele i ka loaʻa ʻana o nā hopena hiʻohiʻona ma nā ʻōlelo hoʻoholo like ʻole.
1.Spot clustering
2. Māhele Spatial
Note: Olelo Hooholopae=13 (100 µm, hema); 7 (50 µm, akau)
3. Hōʻike hōʻailona abundance clustering heatmap
4.Inter-laʻanaʻikepiliʻikepili
