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PacBio-Full-lōʻihi Transcriptome (Non-Reference)
Hoʻokumu ʻia ka paepae Amplicon (16S/18S/ITS) me nā makahiki o ka ʻike i ka microbial diversity project analysis, i loaʻa i ka loiloi kumu maʻamau a me ka loiloi pilikino: uhi ʻia ka loiloi kumu i ka ʻike loiloi kumu o ka noiʻi microbial o kēia manawa, waiwai a piha ka ʻikepili. a hōʻike ʻia nā hopena hoʻopaneʻe ma ke ʻano o nā hōʻike papahana;He ʻokoʻa ka ʻike o ka loiloi pilikino.Hiki ke koho ʻia nā laʻana a hiki ke hoʻonohonoho maʻalahi e like me ka hōʻike loiloi kumu a me ke kumu noiʻi, e ʻike ai i nā koi pilikino.ʻO ka ʻōnaehana hana Windows, maʻalahi a wikiwiki.
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PacBio-Full-lōʻihi Transcriptome (Non-Reference)
Ke lawe nei i ka ʻikepili hoʻokaʻina Isoform i ka Pacific Biosciences (PacBio) ma ke ʻano he hoʻokomo, hiki i kēia App ke ʻike i nā kaʻina transcript holoʻokoʻa (me ka ʻole o ka hui ʻana).Ma ka palapala ʻana i nā kaʻina holoʻokoʻa kūʻē i ka genome kuhikuhi, hiki ke hoʻopaʻa ʻia nā transcripts e nā genes i ʻike ʻia, transcripts, coding regions, etc.ʻO ka loiloi hui pū me ka NGS transcriptome sequencing data e hiki ai i ka hōʻike kikoʻī piha a me ka helu pololei ʻana i ka hōʻike ma ka pae transcript, ka mea e pōmaikaʻi nui ai i ka hōʻike ʻokoʻa a me ka loiloi hana.
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Mea paahana
ʻO BMKCloud kahi papahana bioinformatic alakaʻi e hāʻawi ana i ka hopena hoʻokahi no nā papahana genomic, i hilinaʻi nui ʻia e ka poʻe noiʻi ma nā kahua like ʻole me ka lāʻau lapaʻau, mahiʻai, kaiapuni, a me nā mea ʻē aʻe. , punawai helu, waihona lehulehu, bioinformtic online courses, etc. BMKCloud he mau mea hana bioinformatic i hoohana pinepine ia e like me ka gene annotation, evolutionary genetic tools, ncRNA, data quality control, assembly, alignment, data extraction, mutations, statistics, figure generator, sequence. kālailai, etc.
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RNA liʻiliʻi
ʻO nā RNA liʻiliʻi he ʻano pōkole non-coding RNA me ka lōʻihi o 18-30 nt, me ka miRNA, siRNA a me ka piRNA.Ua hōʻike nui ʻia kēia mau RNA liʻiliʻi i ke komo ʻana i nā kaʻina hana like ʻole e like me ka hoʻohaʻahaʻa ʻana o ka mRNA, ka pāpā ʻana i ka unuhi, ka hoʻokumu ʻana o heterochromatin, a me nā mea ʻē aʻe. ʻO ka papa hana hoʻopaʻa moʻokalaleo e loaʻa ana ka loiloi maʻamau a me ka ʻimi ʻikepili kiʻekiʻe.Ma ke kumu o ka ʻikepili RNA-seq, hiki i ka loiloi maʻamau ke hoʻokō i ka ʻike miRNA a me ka wānana, ka wānana gene target miRNA, annotation a me ka nānā ʻana i ka ʻōlelo.Hiki i ka ʻimi noiʻi kiʻekiʻe ke ʻimi i ka miRNA maʻamau a me ka unuhi ʻana, Venn diagram generation, miRNA and target gene network building.
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NGS-WGS (Illumina/BGI)
ʻO NGS-WGS kahi kahua hoʻopaʻa ʻana i ka genome re-sequencing, i hoʻomohala ʻia ma ke kumu o ka ʻike waiwai ma Biomarker Technologies.Hāʻawi kēia paepae maʻalahi i ka hoʻouna wikiwiki ʻana i kahi kaila hana hoʻopili i hoʻohui ʻia ma ka hoʻonohonoho ʻana i kekahi mau ʻāpana kumu, i kūpono i ka ʻikepili sequencing DNA i hana ʻia mai ka paepae ʻo Illumina a me BGI sequencing platform.Hoʻokomo ʻia kēia paepae ma luna o ka server computing hana kiʻekiʻe, kahi e hāʻawi ai i ka mana o ka nānā ʻana i ka ʻikepili maikaʻi loa i ka manawa pōkole loa.Loaʻa ka ʻimi ʻikepili pilikino ma ke kumu o ka nānā ʻana maʻamau, me ka huli ʻana i nā gene mutated, PCR primer design, etc.
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mRNA(Hōʻike)
ʻO Transcriptome ka loulou ma waena o ka ʻike genetic genomic a me ka proteome o ka hana biological.ʻO ka hoʻoponopono pae transcription ka mea koʻikoʻi a ʻo ke ʻano hoʻoponopono i aʻo nui ʻia o nā meaola.Hiki iā ia ke hoʻonohonoho i ka transcriptome i kēlā me kēia manawa a i ʻole ma lalo o kekahi kūlana, me ka hoʻoholo pololei i ka nucleotide hoʻokahi. laʻana i nā palapala kikoʻī.
I kēia manawa, ua hoʻohana nui ʻia ka ʻenehana sequencing transcriptome i ka agronomy, ka lāʻau lapaʻau a me nā ʻano noiʻi ʻē aʻe, me ka hoʻoponopono ʻana i nā holoholona a me nā mea kanu, ka hoʻoponopono ʻana i ke kaiapuni, ka launa pū ʻana, ka gene localization, species genetic evolution and tumor and genetic disease detection.
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Metagenomics (NGS)
Hoʻolālā ʻia kēia kahua loiloi no ka pana ʻana i ka ʻikepili metagenomic shotgun ma ke kumu o nā makahiki o ka ʻike.Loaʻa ia i ke kahe hana i hoʻohui ʻia me nā ʻano loiloi metagenomics maʻamau e pono ai e pili ana i ka hoʻoili ʻana i ka ʻikepili, nā haʻawina pae ʻano, nā haʻawina pae hana gene, metagenome binning, a me nā mea ʻē aʻe. , ka hoʻonohonoho ʻana i nā ʻāpana, ka hana ʻana i nā kiʻi pilikino, etc.
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LncRNA
ʻO nā RNA non-coding lōʻihi (lncRNA) he ʻano o nā transcripts me ka lōʻihi ma mua o 200 nt, ʻaʻole hiki ke hoʻopaʻa i nā protein.Hōʻike nā hōʻike hōʻuluʻulu e hiki ke hana ka hapa nui o nā lncRNA.Hiki iā mākou ke hōʻike i nā kaʻina o ka lncRNA a me ka hoʻonohonoho ʻana i ka ʻike a alakaʻi iā mākou e ʻike i nā lncRNA me nā hana hoʻoponopono koʻikoʻi.Haʻaheo ʻo BMKCloud e hāʻawi i kā mākou mea kūʻai aku lncRNA sequencing analysis platform e hoʻokō i ka loiloi lncRNA wikiwiki, hilinaʻi a maʻalahi.
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GWAS
ʻO ka Genome-wide association study (GWAS) ka manaʻo e ʻike i nā ʻano ʻano genetic (genotype) e pili ana me nā ʻano kikoʻī (phenotype).Ke noiʻi nei nā haʻawina GWA i nā hōʻailona genetic e hele i ka genome holoʻokoʻa o ka heluna nui o nā kānaka a wānana i nā hui genotype-phenotype ma o ka loiloi helu helu ma ka pae heluna kanaka.Hiki ke ʻike i nā ʻano genetic āpau i ka resequencing holoʻokoʻa.I ka hui pū ʻana me ka ʻikepili phenotypic, hiki ke hana ʻia ʻo GWAS e ʻike i nā SNP pili phenotype, QTL a me nā genes moho, e kākoʻo ikaika ana i ka hoʻohua holoholona/mea kanu hou.ʻO SLAF kahi hoʻolālā hoʻonohonoho genome maʻalahi i hoʻomohala ʻia, e ʻike ana i nā māka hoʻolaha genome-ākea, SNP.Hiki ke hana ʻia kēia mau SNP, ma ke ʻano he molecular genetic markers, no nā haʻawina hui me nā ʻano i kuhikuhi ʻia.He hoʻolālā kumu kūʻai i ka ʻike ʻana i nā ʻano paʻakikī e pili ana i nā ʻano like ʻole.
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Nanopore Ka lōʻihi lōʻihi
ʻO nā isoforms paʻakikī a me nā ʻano like ʻole i loko o nā mea ola he ʻano hana genetic koʻikoʻi no ka hoʻoponopono ʻana i ka hōʻike gene a me ka ʻokoʻa protein.ʻO ka ʻike pololei ʻana i nā hale transcript ke kumu no ka noiʻi hohonu ʻana i nā ʻano hoʻoponopono hōʻike gene.Ua hoʻokō maikaʻi ʻo Nanopore sequencing platform i ka haʻawina transcriptomic i ka pae isoform.Ua hoʻolālā ʻia kēia kahua loiloi e kālailai i ka ʻikepili RNA-Seq i hana ʻia ma ke kahua Nanopore ma ke kumu o ka genome reference, e hoʻokō ai i nā loiloi qualitative a me quantitative ma nā pae gene a me nā pae transcripts.
