ʻO Genome Paʻa ʻo Bacteria
Pono lawelawe
● Loaʻa nā hoʻolālā hoʻonohonoho hoʻonohonoho lehulehu no nā pahuhopu noiʻi like ʻole
● ʻO ka genome piha ka bacteria me ka 0 Gap i hōʻoia ʻia.
● ʻIke loa i ka hui genome bacteria me ka ʻoi aku o 10,000 piha genome i hui ʻia.
● 'Oihana ma hope o ke kū'ai 'ana i ke kāko'o 'oihana hui e ho'okō ana i nā pono noi'i kiko'ī.
Nā kikoʻī lawelawe
| Ke kaʻina ʻanaHoʻolālā | Hale Waihona Puke | Paʻa ka maikaʻi | Kuhi ʻia ka manawa huli |
| Nanopore 100X + Illumina 50X | Nanopore 20K PE150 | 0 Awawa | 30 mau lā |
| PacBio HiFi 30X | PacBio 10K |
Nā loiloi bioinformatics
● Ka hoʻomalu maikaʻi ʻikepili maka
● Hui Genome
● Ke kālailai ʻāpana genome
● Hōʻike hana Gene
● Hoʻohālikelike genomic analysis
Nā Koina Laʻana a me ka Hoʻouna ʻana
Nā Koina Laʻana:
No ka meaNā unuhi DNA:
| ʻAno Laʻana | Ka nui | Hoʻopaʻa ʻana | Maemae |
| Nā unuhi DNA | ＞ 2 μg | ＞ 20 ng/μl | OD260/280= 1.6-2.5 |
No nā laʻana kiko:
| ʻAno laʻana | Manaʻo laʻana lapaʻau | Laʻana mālama a hoʻouna |
| bacteria | E nānā i ka maʻi bacteria ma lalo o ka microscope a e hōʻiliʻili i nā hua bacteria i ko lākou manawa exponential E hoʻololi i ka moʻomeheu bacteria (loaʻa ma kahi o 3-4.5e9 mau keena) i loko o kahi eppendorf 1.5 a i ʻole 2 ml.(E hoomau i ka hau) Centrifuge i ka paipu no 1 min ma 14000 g e hōʻiliʻili i ka hua bacteria a wehe pono i ka supernatant E hoʻopaʻa i ka paipu a hoʻokuʻu i ka hua bacteria i loko o ka nitrogen wai no 30 mau minuke.E mālama i ka paipu i loko o -80 ℃ friji. | E hoʻokuʻu i nā laʻana i loko o ka hau hau no 3-4 mau hola a e mālama i loko o ka wai nitrogen a i ʻole -80 degere a hiki i ka mālama lōʻihi.Pono ka hoʻouna laʻana me ka hau maloʻo. |
Holo Hana Hana
Hāʻawi laʻana
Kūkulu hale waihona puke
Ke kaʻina ʻana
ʻIkepili ʻikepili
Nā lawelawe ma hope o ke kūʻai aku
1.Circos o ka genome bacteria
2. Ka wānana gene coding
3. Ka hoʻohālikelike ʻana i nā genomics: lāʻau phylogenetic
