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ʻIke no ka Transposase-Accessible Chromatin me ke Kiʻekiʻe Throughput Sequencing (ATAC-seq)

ʻIke no ka Transposase-Accessible Chromatin me ke Kiʻi Kiʻekiʻe Throughput Sequencing (ATAC-seq)
  • ʻIke no ka Transposase-Accessible Chromatin me ke Kiʻekiʻe Throughput Sequencing (ATAC-seq)
  • ʻIke no ka Transposase-Accessible Chromatin me ke Kiʻekiʻe Throughput Sequencing (ATAC-seq)
  • ʻIke no ka Transposase-Accessible Chromatin me ke Kiʻekiʻe Throughput Sequencing (ATAC-seq)
  • ʻIke no ka Transposase-Accessible Chromatin me ke Kiʻekiʻe Throughput Sequencing (ATAC-seq)
  • ʻIke no ka Transposase-Accessible Chromatin me ke Kiʻekiʻe Throughput Sequencing (ATAC-seq)

ʻO ATAC-seq kahi ala hoʻokaʻina kiʻekiʻe no ka nānā ʻana i ka hiki ke komo i ka chromatin genome-ākea, he mea nui ia no ka mana epigenetic honua o ka hōʻike gen.Hoʻokomo ʻia nā mea hoʻopili sequencing i nā ʻāpana chromatin ākea e ka hyperactive Tn5 transposase.Ma hope o ka hoʻonui ʻia ʻana o PCR, kūkulu ʻia kahi waihona sequencing.Hiki ke loaʻa nā ʻāpana chromatin hāmama a pau ma lalo o kahi kūlana manawa-manawa kikoʻī, ʻaʻole i kaupalena ʻia i nā wahi paʻa o kahi mea transcription, a i ʻole kekahi ʻāpana histone i hoʻololi ʻia.


Nā kikoʻī lawelawe

Bioinformatics

Nā hopena hōʻike

Pono lawelawe

● Hoʻomākaʻikaʻi ʻana: ʻO ka pipeline kānana ʻoihana e hoʻokō i nā ʻano pono loiloi maʻamau;

● ʻIke Nui: Ua hoʻopau maikaʻi nā haneli o nā papahana i kēia manawa，me nā holoholona a me nā mea kanu.

Nā kikoʻī lawelawe

Papahana: Illumina NovaSeq kahua

ʻAno waihona: ATAC-seq

Hoʻopuka ʻia ka ʻikepili i manaʻo ʻia: ≥20M heluhelu

Nā Koina Laʻana

ʻAno laʻana: Tissues, cell live, a i ʻole cell frozen a pēlā aku

Helu kelepona: ≥ 5×105nā pūnaewele, ʻo ka helu kelepona pololei ke kī i ka holomua o ka hoʻokolohua;

Ke kaumaha o ke kino: ≥ 200mg mea hou;

Koko: ≥ 2 mL

Holo Hana Hana

Laʻana QC

Hoʻolālā hoʻokolohua

hāʻawi laʻana

Hāʻawi laʻana

Hoʻokolohua hoʻokele

Ka unuhi ʻana o DNA

Hoʻomākaukau hale waihona puke

Kūkulu hale waihona puke

Ke kaʻina ʻana

Ke kaʻina ʻana

ʻIkepili ʻikepili

ʻIkepili ʻikepili

Mahope o ke kuai ana

Nā lawelawe ma hope o ke kūʻai aku

  • Mua:
  • Aʻe:

    • 表观-02

    1. Heatmap ma ATAC heluhelu i ka hāʻawi ʻana ma TSS a me nā ʻāpana pili (± 3 kb)

    3（1）2.Distribution o ka māhele chromatin hāmama i nā ʻāpana genome like ʻole

    4(1）

    3.Differential peak kahea ma waena o nā hui

    5(1）

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