Babban ingancin gano alama- Fasahar jeri mai ƙima tana taimakawa SLAF-Seq wajen gano ɗaruruwan dubunnan tags a cikin dukkan kwayoyin halitta.
Low dogara ga kwayoyin halitta- Ana iya amfani da shi ga nau'in ko dai tare da ko ba tare da wani nau'i na genome ba.
Tsarin tsari mai sassauƙa- Single-enzyme, dual-enzyme, multi-enzyme narkewa da nau'ikan enzymes iri-iri, duk ana iya zaɓar su don biyan burin bincike daban-daban ko nau'in.Ana amfani da pre-kimanin a cikin siliki don tabbatar da ƙirar enzyme mafi kyau.
Ingantacciyar narkewar enzymatic- An gudanar da gwaje-gwaje na farko don inganta yanayin, wanda ke sa gwajin na yau da kullun ya tabbata kuma abin dogara.Ingancin tarin juzu'i na iya cimma sama da 95%.
Alamar SLAF da aka rarraba ko'ina- Ana rarraba alamun SLAF a ko'ina cikin duk chromosomes zuwa mafi girma, cimma matsakaicin 1 SLAF a kowace 4 kb.
Ingantacciyar gujewa maimaituwa- Matsakaicin maimaitawa a cikin bayanan SLAF-Seq ya ragu zuwa ƙasa da 5%, musamman a cikin nau'ikan da ke da babban matakin maimaitawa, kamar alkama, masara, da sauransu.
Kyawawan kwarewa-Sama da 2000 rufe ayyukan SLAF-Seq akan ɗaruruwan nau'ikan nau'ikan da ke rufe tsirrai, dabbobi masu shayarwa, tsuntsaye, kwari, ƙwayoyin ruwa, da sauransu.
Gudun aiki na bioinformatic da kansaBMKGENE ne ya haɓaka haɗe-haɗe na aikin bioinformatic don SLAF-Seq don tabbatar da aminci da daidaiton fitarwa na ƙarshe.
Dandalin | Conc.(ng/gl) | Jimlar (ug) | OD260/280 |
Illumina NovaSeq | >35 | >1.6(Juzu'i>15μl) | 1.6-2.5 |
Zurfin jeri: 10X/Tag
Girman Halitta | Shawarar SLAF Tags |
< 500 Mb | 100K ko WGS |
500 Mb- 1 GB | 100 K |
1 Gb-2 Gb | 200 K |
Giant ko hadadden kwayoyin halitta | 300-400K |
Aikace-aikace
| Nasiha Girman Yawan Jama'a
| Dabarun tsarawa da zurfi
| |
Zurfin
| Lambar Tag
| ||
GWAS
| Misalin lamba ≥ 200
| 10X
|
Bisa lafazin girman kwayoyin halitta
|
Juyin Halitta
| Mutanen kowannensu Ƙungiya ≥ 10; jimlar samfurori ≥ 30
| 10X
|
Kwantena: 2 ml centrifuge tube
Don yawancin samfurori, muna ba da shawarar kada a adana a cikin ethanol.
Alamar samfurin: Samfuran suna buƙatar a yi musu lakabi a sarari kuma iri ɗaya ga siffan bayanin samfurin da aka ƙaddamar.
Jirgin ruwa: Busasshen ƙanƙara: Ana buƙatar a haɗa samfuran a cikin jakunkuna da farko kuma a binne su a bushe-kankara.
1. Kididdigar sakamakon taswira
2. Ci gaban alamar SLAF
3. Bambance-bambancen bayanin
Shekara | Jarida | IF | Take | Aikace-aikace |
2022 | Sadarwar yanayi | 17.694 | Tushen genomic na giga-chromosomes da giga-genome na itacen peony Paeonia ostii | SLF-GWAS |
2015 | Sabon Likitan Halitta | 7.433 | Sawun cikin gida yana kafa yankuna masu mahimmancin agronomic a cikin waken soya | SLF-GWAS |
2022 | Jaridar Babban Bincike | 12.822 | G. hirsutum na Gossypium barbadense mai faɗin ɗan adam bayyana loci mafi girma don haɓaka ingancin fiber auduga da yawan amfanin ƙasa lokaci guda halaye | SLAF-Genetics na Juyin Halitta |
2019 | Shuka Kwayoyin Halitta | 10.81 | Binciken Al'umma na Al'umma da Majalisar De Novo sun Bayyana Asalin Weedy Shinkafa a matsayin Wasan Juyin Halitta | SLAF-Genetics na Juyin Halitta |
2019 | Halitta Halitta | 31.616 | Tsarin kwayoyin halitta da bambancin kwayoyin halittar irin kifi na kowa, Cyprinus carpio | SLAF-Haɗin taswira |
2014 | Halitta Halitta | 25.455 | Halin halittar gyada da aka noma yana ba da haske game da legume karyotypes, polyploid juyin halitta da amfanin gona domestication. | SLAF-Haɗin taswira |
2022 | Jaridar Biotechnology Journal | 9.803 | Gane ST1 yana bayyana zaɓin da ya haɗa da ƙwanƙwasa ƙwayoyin halittar iri da abun da ke cikin mai a lokacin gidan waken suya | SLF-Marker ci gaba |
2022 | Jaridar Duniya ta Kimiyyar Kwayoyin Halitta | 6.208 | Ganewa da Ci gaban Alamar DNA don Alkama-Leymus mollis 2Ns (2D) Sauya Disomic Chromosome | SLF-Marker ci gaba |