Rage Matsayin Bisulfite Sequencing (RRBS)
Ƙayyadaddun Sabis
Platform: dandalin NovaSeq, PE150
Nau'in ɗakin karatu: 200-400bp saka bisulfite da aka kula da ɗakin karatu na DNA
Dabarar jeri: Haɗa-Ƙarshen 150 bp
Shawarar fitar da bayanai: 10 Gb raw bayanai/samfuri
Samfuran Bukatun
Yawan DNA: ≥ 2ug
Halin DNA: ≥ 20ng/μl.
Tsafta: OD260/280 = 1.8 zuwa 2.0 ba tare da lalacewa ko gurɓataccen RNA ba.
Binciken Bioinformatics
Gudun Aikin Sabis
Samfurin bayarwa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
a.Ƙididdiga daidaitawar kwayoyin halitta
Kididdigar karatun taswira
Sƙididdiga na jerin zurfafawa da ɗaukar hoto na rukunin C
Atara ɗaukar hoto
Digestion yadda ya dace na kowane samfurin
b.Gano wurin methylation
Jerin matakan methylation a rukunin C
BS tuba
Methylated cytosine
Rarraba matakin methylation
c.Taswirar methylation
Gfadi-fadi
Genom element
d.Kwatanta matakan methylation tsakanin samfurori
PDaidaitawar iska na CG methylation
PCA-CG methylation
CƘaddamar da CG methylation
d.Daban-daban methylation bincike
SBayanan Bayani na DMR
Rarraba tsawon DMR
Hcin taswira
DRarraba MR a yankin da aka yi niyya
