Tsarin RNA Prokaryotic

Jerin Prokaryotic RNA da aka Fitar da Hoton

Sequencing na Prokaryotic RNA yana amfani da jerin tsararraki na gaba (NGS) don bayyana kasancewar da adadin RNA a wani ɗan lokaci, ta hanyar nazarin canjin salon salula.Sequencing na kamfaninmu na prokaryotic RNA, musamman yana nufin prokaryotes tare da kwayoyin halitta, yana ba ku bayanan bayanan rubutu, nazarin tsarin kwayoyin halitta, da sauransu. An yi amfani da shi sosai ga bincike na kimiyya na asali, bincike da ci gaban ƙwayoyi, da ƙari.

Platform: Illumina NovaSeq Platform

Tuntube Mu

Cikakkun Sabis

Ƙayyadaddun Sabis

Platform: Illumina NovaSeq dandamali, PE150

Nau'in ɗakin karatu: 350bp saka ɗakin karatu na cDNA (ya danganta da mafi girman rarraba)

Dabarar jeri: Haɗa-Ƙarshen 150 bp

Adadin bayanai: 2 Gb raw data/samfuri

Bioinformatics

(1) Sequencing data ingancin iko: Nucleotide rarraba, Tushen ingancin rarraba, rRNA rabo kima;

(2) Binciken jeri-jeri: Ƙididdiga sakamakon daidaitawa, Cikewa na jeri, Rufe jerin;

(3) Bayanin bayanan kwayoyin halitta (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Binciken Magana: Rarraba magana (Tare da samfurori guda biyu ko fiye), Nazarin magana tsakanin samfurori (bincike na Venn, Binciken Daidaitawa, PCA bincike);

(5) Binciken maganganu daban-daban (Tare da samfurori biyu ko fiye);

(6) Binciken saiti na Gene: Binciken Venn, Binciken Cluster, Nazarin bayanin aiki (COG, GO, KEGG), Binciken haɓaka aikin aiki (GO, KEGG), jadawali haɓaka aikin haɓakawa, nazarin Ipath;

(7) sRNA bincike: sRNA tsinkaya, sRNA annotation (Blast, sRNAMap, sRNATarBase, SIPHT, Rfam), sRNA tsarin sakandare tsinkaya, sRNA manufa tsinkaya;

(8) Binciken tsarin kwafi: Binciken Operon, Rubutu farawa da ƙarshen hasashen shafukan yanar gizo, UTR annotation da bincike, Hasashen jerin abubuwan haɓakawa, SNP/InDel bincike (SNP/InDel annotation, SNP/InDel rarraba yanki, SNP statistics).