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Single-nucleus RNA Sequencing
Ci gaba a cikin ɗaukan tantanin halitta guda ɗaya da dabarar ginin ɗakin karatu na ɗaiɗaikun haɗe tare da babban tsarin aiki yana ba da damar nazarin bayanin kwayoyin halitta akan tushen tantanin halitta.Yana ba da damar zurfafa da cikakken bincike na tsarin akan hadaddun yawan adadin tantanin halitta, wanda a cikinsa ya fi nisantar rufe nau'ikan halittarsu ta hanyar ɗaukar matsakaicin dukkan ƙwayoyin.
Duk da haka, wasu sel ba su dace a sanya su su zama dakatarwar tantanin halitta guda ɗaya ba, don haka wasu hanyoyin shirye-shiryen samfurin - ana buƙatar cirewar tsakiya daga kyallen takarda, wato, tsakiya ana fitar da su kai tsaye daga kyallen takarda ko tantanin halitta kuma an shirya su zuwa dakatarwar tsakiya guda ɗaya don guda ɗaya- jerin kwayoyin halitta.
BMK yana ba da sabis na jeri na 10x Genomics ChromiumTM tushen RNA guda tantanin halitta.An yi amfani da wannan sabis ɗin sosai a cikin binciken akan binciken da ke da alaƙa da cututtuka, irin su bambance-bambancen ƙwayoyin cuta, nau'in ƙwayar cuta, haɓaka nama, da sauransu.
Na'urar kwafin sararin samaniya: 10 × Genomics
Platform: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
Fassarar bayanan sararin samaniya yana tsaye a kan gaba na ƙirƙira ƙirar kimiyya, yana ƙarfafa masu bincike don zurfafa cikin ƙayyadaddun tsarin bayyanar cututtuka a cikin kyallen takarda yayin da suke kiyaye yanayin sararin samaniya.Tsakanin dandamali daban-daban, BMKGene ya haɓaka BMKManu S1000 Spatial Transcriptome Chip, yana alfahari daingantaccen ƙudurina 5µM, isa ga kewayon subcellular, da kunnawasaitunan ƙuduri masu yawa.Guntuwar S1000, wacce ke nuna kusan tabo miliyan 2, tana ɗaukar microwells ɗin da aka lulluɓe tare da beads ɗin da aka ɗora tare da ɓangarorin ɗaukar hoto.An shirya ɗakin karatu na cDNA, wanda aka wadatar tare da lambobin sirri, daga guntuwar S1000 kuma daga baya aka yi ta kan dandalin Illumina NovaSeq.Haɗuwa da samfuran barcoded na sararin samaniya da UMI suna tabbatar da daidaito da ƙayyadaddun bayanan da aka samar.Babban sifa na guntu na BMKManu S1000 ya ta'allaka ne a cikin iyawar sa, yana ba da saitunan ƙuduri masu yawa waɗanda za'a iya daidaita su zuwa kyallen takarda daban-daban da matakan daki-daki.Wannan daidaitawar tana sanya guntu a matsayin zaɓi na musamman don nazarin kwafi daban-daban na sararin samaniya, yana tabbatar da madaidaicin tari tare da ƙaramar amo.
Yin amfani da guntu na BMKManu S1000 da sauran fasahohin fassarar sararin samaniya, masu bincike za su iya samun kyakkyawar fahimta game da tsarin sararin samaniya na sel da kuma hadaddun hulɗar kwayoyin halitta da ke faruwa a cikin kyallen takarda, suna ba da haske mai mahimmanci game da hanyoyin da ke tattare da tsarin ilimin halitta a cikin fage da dama, ciki har da oncology, neuroscience, ilmin halitta na ci gaba, rigakafi da nazarin halittu.
Platform: BMKManu S1000 guntu da Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
Fassarar fasikanci fasaha ce mai yanke hukunci wacce ke ba masu bincike damar bincika tsarin maganganun kwayoyin halitta a cikin kyallen takarda yayin da suke kiyaye yanayin sararin samaniya.Ɗayan dandamali mai ƙarfi a cikin wannan yanki shine 10x Genomics Visium haɗe tare da jerin Illumina.Ka'idar 10X Visium ta ta'allaka ne akan guntu na musamman tare da yankin da aka keɓe inda aka sanya sassan nama.Wannan yanki na kama yana ƙunshe da ɓangarorin ɓangarorin, kowanne ya yi daidai da wani wuri na musamman a cikin nama.Kwayoyin RNA da aka kama daga nama ana yi musu lakabi da kebantattun abubuwan gano kwayoyin halitta (UMIs) yayin aiwatar da rubutun baya.Waɗannan tabobin da aka ƙera da UMI suna ba da damar taswirar taswirar sararin samaniya da ƙididdige yawan magana a cikin ƙudurin tantai ɗaya.Haɗuwa da samfuran barcoded na sararin samaniya da UMI suna tabbatar da daidaito da ƙayyadaddun bayanan da aka samar.Ta amfani da wannan fasaha ta Spatial Transcriptomics, masu bincike za su iya samun zurfin fahimta game da tsarin sararin samaniya na sel da hadaddun hulɗar kwayoyin da ke faruwa a cikin kyallen takarda, suna ba da haske mai mahimmanci game da hanyoyin da ke tattare da tsarin ilimin halitta a fannoni da yawa, ciki har da oncology, neuroscience, ilmin halitta na ci gaba, rigakafi. , da kuma nazarin halittu.
Platform: 10X Genomics Visium da Illumina NovaSeq
