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BSA
Dandali mai girma Segregant Analysis yana ƙunshe da daidaitaccen bincike na mataki ɗaya da bincike na gaba tare da saitin siga na musamman.BSA wata dabara ce da ake amfani da ita don gano alamomin kwayoyin halitta masu alaƙa da sauri.Babban tsarin aiki na BSA ya ƙunshi: 1. zabar ƙungiyoyi biyu na mutane waɗanda ke da ƙima sosai;2. hada DNA, RNA ko SLAF-seq (Biomarker ya Haɓaka) na kowane ɗaiɗai don samar da nau'in DNA guda biyu;3. gano jerin bambance-bambancen da aka saba da kwayar halitta ko tsakanin, 4. tsinkaya dan takarar da aka haɗa yankuna ta ED da SNP-index algorithm;5. Binciken aiki da haɓakawa akan kwayoyin halitta a yankunan yan takara, da dai sauransu. Ƙarin ma'adinai na ci gaba a cikin bayanai ciki har da gwajin alamar kwayoyin halitta da kuma zane-zane.
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Amplicon (16S/18S/ITS)
Amplicon (16S / 18S / ITS) dandamali an haɓaka shi tare da shekaru na gwaninta a cikin nazarin ayyukan bambance-bambancen microbial, wanda ya ƙunshi daidaitaccen bincike na asali da bincike na keɓaɓɓu: bincike na asali ya ƙunshi babban abun ciki na bincike na bincike na microbial na yanzu, abun ciki na bincike yana da wadata kuma cikakke. kuma ana gabatar da sakamakon bincike ta hanyar rahotannin aikin;Abubuwan da ke cikin keɓaɓɓen bincike sun bambanta.Za'a iya zaɓar samfurori kuma ana iya saita sigogi cikin sassauƙa bisa ga ainihin rahoton bincike da manufar bincike, don gane keɓaɓɓen buƙatun.Tsarin aiki na Windows, mai sauƙi da sauri.
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Juyin Halitta
Juyin Halitta sabis ne mai cike da jeri wanda aka ƙera don samar da cikakkiyar fassarar bayanan juyin halitta na kayan da aka bayar dangane da bambance-bambancen kwayoyin halitta, gami da SNPs, InDels, SVs da CNVs.Yana bayar da duk mahimman bincike da ake buƙata don kwatanta sauye-sauyen juyin halitta da sifofin kwayoyin halitta na yawan jama'a, kamar tsarin yawan jama'a, bambancin jinsin halitta, dangantakar phylogeny, da dai sauransu. Hakanan ya ƙunshi nazari akan kwararar kwayoyin halitta, wanda ke ba da damar kimanta ingancin girman yawan jama'a, lokacin rarrabuwa.
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Kwatanta Genomics
Kwatankwacin ilimin genomics a zahiri yana nufin kwatanta cikakken jerin kwayoyin halitta da sigar nau'ikan nau'ikan nau'ikan nau'ikan.Wannan horon yana nufin bayyanar da juyin halittar nau'in halitta, aikin kwayoyin halitta, tsarin sarrafa kwayoyin halitta a matakin kwayoyin halitta ta hanyar gano tsarin tsari da abubuwan da aka kiyaye ko bambanta a cikin nau'ikan nau'ikan daban-daban.Nazari na kwatankwacin ilimin genomics na yau da kullun ya haɗa da nazari a cikin dangin jinsin halitta, haɓakar juyin halitta, kwafin kwayoyin halitta gabaɗaya, matsa lamba, da sauransu.
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Juyin Halitta
An kafa dandalin tantance yawan jama'a da juyin halitta bisa ga dimbin gogewa da aka tara a cikin tawagar BMK R&D tsawon shekaru.Kayan aiki ne mai sauƙin amfani musamman ga masu bincike waɗanda ba su da fifiko a cikin bioinformatics.Wannan dandali yana ba da damar ainihin ilimin halittar ɗan adam da ke da alaƙa da bincike na asali wanda ya haɗa da ginin bishiyar phylogenetic, bincike na rashin daidaituwa, kimanta bambancin kwayoyin halitta, nazarin share fage, nazarin dangi, PCA, nazarin tsarin yawan jama'a, da sauransu.
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Hi-C tushen Genome Assembly
Hi-C wata hanya ce da aka ƙera don ɗaukar daidaitawar chromosome ta hanyar haɗa ma'amala ta tushen kusanci da babban tsarin aiwatarwa.An yi imanin ƙarfin waɗannan hulɗar yana da alaƙa mara kyau tare da nisa ta jiki akan chromosomes.Saboda haka, bayanan Hi-C na iya jagorantar tari, oda da daidaita jerin abubuwan da aka haɗa a cikin daftarin kwayoyin halitta da kuma rataye su akan takamaiman adadin chromosomes.Wannan fasaha tana ba da ƙarfin taro na matakin chromosome ba tare da taswirar tushen yawan jama'a ba.Kowane genome guda ɗaya yana buƙatar Hi-C.
Platform: Illumina NovaSeq Platform / DNBSEQ
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Tsarin Shuka/ Dabba De Novo Genome Sequencing
De Novosequencing na nufin gina wani nau'i 'dukan kwayoyin halitta ta hanyar amfani da fasahohin jeri, misali PacBio, Nanopore, NGS, da dai sauransu, in babu kwayar halitta.Haɓakawa mai ban sha'awa a cikin tsayin karantawa na fasahar jerin tsararrun ƙarni na uku ya kawo sabbin damammaki a haɗa hadaddun kwayoyin halitta, kamar waɗanda ke da babban heterozygosity, babban rabo na yankuna masu maimaitawa, polyploids, da sauransu. warware abubuwa masu maimaitawa, yankuna tare da abubuwan da ke cikin GC mara kyau da sauran yankuna masu rikitarwa.
Platform: PacBio Sequel II/Nanopore PromethION P48/ Illumina NovaSeq Platform
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Tsarin Dan Adam Gabaɗaya Exome
Ana ɗaukarsa gabaɗaya exome sequencing (WES) azaman dabarar jeri mai tsada don gano maye gurbi masu haifar da cututtuka.Kodayake exons kawai suna ɗaukar kusan 1.7% na dukkan kwayoyin halitta, yana wakiltar bayanin martabar ayyukan furotin kai tsaye.A cikin kwayoyin halittar dan adam, an bayar da rahoton cewa fiye da kashi 85% na sauye-sauyen da ke da alaka da cututtuka suna faruwa a yankin da ake kira furotin.
BMKGENE yana ba da cikakkun ayyuka masu sassaucin ra'ayi na ɗan adam gabaɗayan exome sequencing tare da dabaru daban-daban na kamawa don cimma burin bincike daban-daban.
Platform: Illumina NovaSeq Platform
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Takamaiman-Locus Amplified Sequencing Sequencing (SLAF-Seq)
Ƙwararren ƙwayar cuta mai girma, musamman a kan yawan jama'a, wani muhimmin mataki ne a cikin nazarin ƙungiyoyin kwayoyin halitta, wanda ke ba da tushen kwayoyin halitta don gano aikin kwayoyin halitta, nazarin juyin halitta, da dai sauransu. Maimakon zurfin sake sake tsarin kwayoyin halitta, rage yawan wakilcin kwayoyin halitta (RRGS). ) an gabatar da shi don rage farashin jeri akan kowane samfuri, yayin da ake kiyaye ingantaccen aiki akan gano alamun kwayoyin halitta.Ana samun wannan galibi ta hanyar ciro ɓangarorin taƙaitawa a cikin kewayon girman da aka ba, wanda ake kiran sunan ɗakin karatu na wakilci (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) dabara ce ta ci gaba da kai don SNP genotyping tare da ko ba tare da genome na tunani ba.
Platform: Illumina NovaSeq Platform -
Illumina da BGI
Fasahar sequencing na Illumina, dangane da Sequencing by Synthesis (SBS), ƙirƙira ce ta NGS ta duniya wacce ke da alhakin samar da sama da kashi 90% na bayanan jeri na duniya.Ka'idar SBS ta ƙunshi ɗaukar hoto mai lakabi mai jujjuyawa kamar yadda aka ƙara kowane dNTP, kuma daga baya an raba shi don ba da damar haɗa tushe na gaba.Tare da duk dNTPs guda huɗu masu jujjuyawa masu ɗaure da ke akwai a cikin kowane zagayowar zagayowar, gasa ta yanayi tana rage son haɗawa.Wannan fasaha mai jujjuyawar tana goyan bayan dakunan karatu guda-ɗaya da masu haɗa-zuwa-ƙarshe, suna ba da kewayon aikace-aikacen kwayoyin halitta.Illumina sequencing's high-throughput capabilities and madaidaicin sanya shi a matsayin ginshiƙi a cikin binciken kimiyyar halittu, ƙarfafa masana kimiyya don buɗe ɓarna na kwayoyin halitta tare da cikakkun bayanai da inganci.
DNBSEQ, wanda BGI ta haɓaka, wata sabuwar fasaha ce ta NGS wacce ta yi nasarar rage raguwar farashin jeri da haɓaka kayan aiki.Shirye-shiryen dakunan karatu na DNBSEQ ya ƙunshi rarrabuwar DNA, shirye-shiryen ssDNA da haɓaka da'ira don samun DNA nanoballs (DNB).Ana ɗora waɗannan a kan ƙaƙƙarfan wuri kuma daga baya ana yin su ta hanyar haɗakar Binciken-Anchor Synthesis (cPAS).
Sabis ɗin jerin laburaren da aka yi da aka riga aka yi yana sauƙaƙe abokan ciniki wajen shirya dakunan karatu daga mabambanta (mRNA, gabaɗayan kwayoyin halitta, amplicon, da sauransu).Daga baya, waɗannan ɗakunan karatu za a iya jigilar su zuwa cibiyoyin jerin abubuwan mu don kula da inganci da tsari a dandamalin Illumina ko BGI.
