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Chromatin Immunoprecipitation Sequencing (ChiP-seq)
ChIP-Seq yana ba da faffadan bayanan kwayoyin halittar DNA don gyare-gyaren tarihi, abubuwan rubutawa, da sauran sunadaran da ke da alaƙa da DNA.Yana haɗuwa da zaɓi na chromatin immuno-hazo (ChiP) don dawo da takamaiman rukunin furotin-DNA, tare da ikon jerin tsararru na gaba (NGS) don babban tsarin aiwatarwa na DNA da aka gano.Bugu da ƙari, saboda an dawo da rukunin furotin-DNA daga sel masu rai, ana iya kwatanta wuraren ɗaure a nau'ikan tantanin halitta da kyallen takarda, ko ƙarƙashin yanayi daban-daban.Aikace-aikace sun bambanta daga ƙa'idodin rubuce-rubuce zuwa hanyoyin haɓakawa zuwa hanyoyin cututtuka da ƙari.
Platform: Illumina NovaSeq Platform
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Tsarin Metagenomic -NGS
Metagenome yana nufin tarin tarin kwayoyin halitta na gauraya al'umma, kamar muhalli metagenome, mutum metagenome, da dai sauransu. Yana dauke da kwayoyin halittun da ake nomawa da wadanda ba za a iya nomawa ba.Tsarin metagenomic kayan aiki ne na kwayoyin halitta da ake amfani da shi don nazarin gaurayen kayan halitta da aka samo daga samfuran muhalli, wanda ke ba da cikakkun bayanai game da bambancin nau'in da yawa, tsarin yawan jama'a, dangantakar phylogenetic, kwayoyin aiki da hanyar sadarwa tare da abubuwan muhalli.
Dandalin:Illumina NovaSeq Platform
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Tsarin Metagenomic-Nanopore
Metagenomics wani kayan aiki ne na kwayoyin halitta da ake amfani dashi don nazarin abubuwan da aka haɗe da kwayoyin halitta da aka samo daga samfurori na muhalli, wanda ke ba da cikakkun bayanai game da bambancin nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i) nau'i-nau'i-nau'i na kwayoyin da ake amfani da su don nazarin abubuwan da suka shafi muhalli,da sauransu. zuwa nazarin metagenomic.Fitaccen aikin sa a cikin tsayin karantawa ya inganta binciken metagenomic na rafi, musamman taron metagenome.Yin amfani da fa'idodin tsayin karantawa, nazarin metagenomic na tushen Nanopore yana iya samun ƙarin ci gaba da taro idan aka kwatanta da abubuwan metagenomics na harbi.An buga cewa Nanopore na tushen metagenomics sun sami nasarar haifar da cikakkun kuma rufaffiyar kwayoyin halittar kwayoyin cuta daga microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
Dandalin:Nanopore PromethION P48
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Gabaɗayan jerin abubuwan genome bisulfite
DNA methylation a matsayi na biyar a cikin cytosine (5-mC) yana da tasiri mai mahimmanci akan maganganun kwayoyin halitta da aikin salula.An haɗu da tsarin methylation mara kyau tare da yanayi da cututtuka da yawa, kamar ciwon daji.WGBS ya zama ma'auni na zinariya don nazarin methylation-fadi na genome a ƙudurin tushe guda ɗaya.
Platform: Illumina NovaSeq Platform
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Ƙididdiga don Canjawa-Sarfafa Chromatin tare da Babban Sabis na Sabis (ATAC-seq)
ATAC-seq hanya ce mai mahimmanci don yin nazari akan samun damar chromatin mai fa'ida ga kwayoyin halitta, wanda ke da mahimmanci ga kula da epigenetic na duniya na maganganun kwayoyin halitta.Ana shigar da adaftan jeri cikin buɗaɗɗen yankuna na chromatin ta hanyar transposase mai ƙarfi Tn5.Bayan haɓakawa na PCR, ana gina ɗakin karatu na jerin abubuwa.Ana iya samun duk yankuna na chromatin da ke buɗe ƙarƙashin takamaiman yanayin lokacin sarari, ba kawai iyakance ga wuraren ɗaure na abin rubutu ba, ko wani yanki da aka gyara na tarihi.
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16S/18S/ITS Amplicon Sequencing-PacBio
Rukunin da ke kan 16S da 18S rRNA wanda ya ƙunshi yankuna biyun da aka kiyaye su sosai da kuma masu saurin canzawa shine cikakken hoton yatsa na kwayoyin halitta don gano kwayoyin prokaryotic da eukaryotic.Shan amfani da sequencing, wadannan amplicons za a iya niyya dangane da kiyaye sassa da kuma hyper-canzawa yankuna za a iya cikakken hali ga microbial ganewa na taimaka wa nazarin rufe microbial bambancin bincike, taxonomy, phylogeny, da dai sauransu Single-kwayoyin real-lokaci (SMRT). Tsarin tsarin dandamali na PacBio yana ba da damar samun ingantaccen dogon karantawa, wanda zai iya rufe amplicons masu tsayi (kimanin 1.5 Kb).Faɗin ra'ayi game da filin kwayoyin halitta ya inganta ƙudiri sosai a cikin bayanin nau'in ƙwayoyin cuta ko fungi.
Dandalin:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing da nufin bayyana phylogeny, haraji, da nau'i mai yawa a cikin microbial al'umma ta hanyar binciken PCR kayayyakin na kiyaye gida alamomin kwayoyin halitta wanda ya ƙunshi duka biyu hira sosai da kuma hypervariable sassa.Gabatar da waɗannan cikakkiyar sawun yatsa na ƙwayoyin cuta ta Woeses et al, (1977) yana ba da damar keɓance bayanan microbiome mara amfani.Jeri na 16S (kwayoyin cuta), 18S (fungi) da na waje da aka rubuta na ciki (ITS, fungi) yana ba da damar gano nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan iri biyu ne, da nau'ikan da ba a san su ba.Wannan fasaha ta zama kayan aiki da yawa da ake amfani da su kuma babban kayan aiki don gano nau'ikan ƙwayoyin cuta daban-daban a cikin yanayi daban-daban, kamar bakin ɗan adam, hanji, najasa, da sauransu.
Dandalin:Illumina NovaSeq Platform
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Bacterial da Fungal Dukan Halittun Halittu Sake-Sequencing
Bacterial da fungal dukan kwayoyin halittar sake-sequencing kayan aiki ne mai mahimmanci don kammala kwayoyin halittar ƙwayoyin cuta da fungi da aka sani, da kuma kwatanta kwayoyin halitta masu yawa ko yin taswirar kwayoyin halitta na sababbin kwayoyin halitta.Yana da matukar muhimmanci a jera dukkan kwayoyin halittar kwayoyin cuta da fungi domin samar da ingantattun kwayoyin halittar kwayoyin halitta, don yin gano kwayoyin halitta da sauran nazarin kwayoyin halittar kwatance.
Platform: Illumina NovaSeq Platform
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PacBio-Full-tsawon 16S/18S/ITS Tsarin Amplicon
Amplicon (16S / 18S / ITS) dandamali an haɓaka shi tare da shekaru na gwaninta a cikin nazarin ayyukan bambance-bambancen microbial, wanda ya ƙunshi daidaitaccen bincike na asali da bincike na keɓaɓɓu: bincike na asali ya ƙunshi babban abun ciki na bincike na bincike na microbial na yanzu, abun ciki na bincike yana da wadata kuma cikakke. kuma ana gabatar da sakamakon bincike ta hanyar rahotannin aikin;Abubuwan da ke cikin keɓaɓɓen bincike sun bambanta.Za'a iya zaɓar samfurori kuma ana iya saita sigogi cikin sassauƙa bisa ga ainihin rahoton bincike da manufar bincike, don gane keɓaɓɓen buƙatun.Tsarin aiki na Windows, mai sauƙi da sauri.
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Rubutun Rubutun-cikakken-PacBio (Ba Nasiha ba)
Ɗaukar bayanan jeri na Biosciences na Pacific (PacBio) Isoform a matsayin shigarwa, wannan App yana da ikon gano jerin cikakkun bayanai na rubutun (ba tare da taro ba).Ta hanyar yin taswirar cikakken tsayin daka akan kwayoyin halitta, za a iya inganta kwafin ta hanyar sanannun kwayoyin halitta, kwafi, yanki, da sauransu.Binciken haɗin gwiwa tare da bayanan bayanan kwafi na NGS yana ba da damar ƙarin cikakkun bayanai da ƙarin ƙididdige ƙididdigewa a cikin magana a matakin kwafi, wanda ya fi fa'ida fa'ida ta ƙasa daban-daban da bincike na aiki.
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Rage Matsayin Bisulfite Sequencing (RRBS)
Binciken methylation na DNA ya kasance batu mai zafi a cikin bincike na cututtuka, kuma yana da alaƙa da alaƙa da maganganun kwayoyin halitta da halaye masu kama-da-wane.RRBS hanya ce mai kyau, inganci da tattalin arziki don binciken DNA methylation.Haɓakawa na masu haɓakawa da yankunan tsibirin CpG ta hanyar ɓarnawar enzymatic (Msp I), haɗe tare da jerin Bisulfite, yana ba da babban ƙudurin gano methylation na DNA.
Platform: Illumina NovaSeq Platform
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Prokaryotic mRNA jerin
Tsarin mRNA yana ba da ikon cikakken bayanin duk bayanan mRNA a cikin sel a ƙarƙashin takamaiman yanayi.Wannan fasaha mai yanke-yanke tana aiki azaman kayan aiki mai ƙarfi, yana buɗe bayanan bayanan ƙwayoyin cuta masu rikitarwa, tsarin halittar halitta, da hanyoyin ƙwayoyin ƙwayoyin cuta masu alaƙa da nau'ikan hanyoyin nazarin halittu.An karɓe shi sosai a cikin bincike na asali, bincike na asibiti, da haɓaka magunguna, jerin mRNA yana ba da haske game da rikitattun kuzarin salon salula da ka'idojin kwayoyin halitta.Samfurin samfurin mu na prokaryotic mRNA an keɓance shi don kwafin prokaryotic, wanda ya haɗa da raguwar rRNA da shirye-shiryen ɗakin karatu na jagora.
Platform: Illumina NovaSeq X
