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Single-nucleus RNA Sequencing
Ci gaba a cikin ɗaukan tantanin halitta guda ɗaya da dabarar ginin ɗakin karatu na ɗaiɗaikun haɗe tare da babban tsarin aiki yana ba da damar nazarin bayanin kwayoyin halitta akan tushen tantanin halitta.Yana ba da damar zurfafa da cikakken bincike na tsarin akan hadaddun yawan adadin tantanin halitta, wanda a cikinsa ya fi nisantar rufe nau'ikan halittarsu ta hanyar ɗaukar matsakaicin dukkan ƙwayoyin.
Duk da haka, wasu sel ba su dace a sanya su su zama dakatarwar tantanin halitta guda ɗaya ba, don haka wasu hanyoyin shirye-shiryen samfurin - ana buƙatar cirewar tsakiya daga kyallen takarda, wato, tsakiya ana fitar da su kai tsaye daga kyallen takarda ko tantanin halitta kuma an shirya su zuwa dakatarwar tsakiya guda ɗaya don guda ɗaya- jerin kwayoyin halitta.
BMK yana ba da sabis na jeri na 10x Genomics ChromiumTM tushen RNA guda tantanin halitta.An yi amfani da wannan sabis ɗin sosai a cikin binciken akan binciken da ke da alaƙa da cututtuka, irin su bambance-bambancen ƙwayoyin cuta, nau'in ƙwayar cuta, haɓaka nama, da sauransu.
Na'urar kwafin sararin samaniya: 10 × Genomics
Platform: Illumina NovaSeq Platform
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Tsarin Tsiro/Dabbobi Gabaɗayan Halitta
Dukkanin tsarin sake-sake-tsarin kwayoyin halitta, wanda kuma aka sani da WGS, yana ba da damar bayyanar da sauye-sauye na yau da kullun da na yau da kullun akan dukkan kwayoyin halittar da suka hada da Single Nucleotide Polymorphism (SNP), Sharewa Shiga (InDel), Bambancin Tsarin (SV), da Bambancin Lamba Kwafi (CNV). ).SVs sun ƙunshi babban ɓangare na tushen bambancin fiye da SNPs kuma suna da tasiri mafi girma akan kwayoyin halitta, wanda ke da tasiri mai mahimmanci akan rayayyun halittu.Tsawon karatu mai tsawo yana ba da damar ƙarin ainihin gano manyan gutsuttsura da bambance-bambance masu rikitarwa saboda dogon karantawa yana ba da sauƙin hayewar chromosomal akan yankuna masu rikitarwa kamar maimaita tandem, yankuna masu wadatar GC/AT, da yankuna masu saurin canzawa.
Platform: Illumina, PacBio, Nanopore
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BMKMANU S1000 Spatial Transcriptome
Fassarar bayanan sararin samaniya yana tsaye a kan gaba na ƙirƙira ƙirar kimiyya, yana ƙarfafa masu bincike don zurfafa cikin ƙayyadaddun tsarin bayyanar cututtuka a cikin kyallen takarda yayin da suke kiyaye yanayin sararin samaniya.Tsakanin dandamali daban-daban, BMKGene ya haɓaka BMKManu S1000 Spatial Transcriptome Chip, yana alfahari daingantaccen ƙudurina 5µM, isa ga kewayon subcellular, da kunnawasaitunan ƙuduri masu yawa.Guntuwar S1000, wacce ke nuna kusan tabo miliyan 2, tana ɗaukar microwells ɗin da aka lulluɓe tare da beads ɗin da aka ɗora tare da ɓangarorin ɗaukar hoto.An shirya ɗakin karatu na cDNA, wanda aka wadatar tare da lambobin sirri, daga guntuwar S1000 kuma daga baya aka yi ta kan dandalin Illumina NovaSeq.Haɗuwa da samfuran barcoded na sararin samaniya da UMI suna tabbatar da daidaito da ƙayyadaddun bayanan da aka samar.Babban sifa na guntu na BMKManu S1000 ya ta'allaka ne a cikin iyawar sa, yana ba da saitunan ƙuduri masu yawa waɗanda za'a iya daidaita su zuwa kyallen takarda daban-daban da matakan daki-daki.Wannan daidaitawar tana sanya guntu a matsayin zaɓi na musamman don nazarin kwafi daban-daban na sararin samaniya, yana tabbatar da madaidaicin tari tare da ƙaramar amo.
Yin amfani da guntu na BMKManu S1000 da sauran fasahohin fassarar sararin samaniya, masu bincike za su iya samun kyakkyawar fahimta game da tsarin sararin samaniya na sel da kuma hadaddun hulɗar kwayoyin halitta da ke faruwa a cikin kyallen takarda, suna ba da haske mai mahimmanci game da hanyoyin da ke tattare da tsarin ilimin halitta a cikin fage da dama, ciki har da oncology, neuroscience, ilmin halitta na ci gaba, rigakafi da nazarin halittu.
Platform: BMKManu S1000 guntu da Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
Fassarar fasikanci fasaha ce mai yanke hukunci wacce ke ba masu bincike damar bincika tsarin maganganun kwayoyin halitta a cikin kyallen takarda yayin da suke kiyaye yanayin sararin samaniya.Ɗayan dandamali mai ƙarfi a cikin wannan yanki shine 10x Genomics Visium haɗe tare da jerin Illumina.Ka'idar 10X Visium ta ta'allaka ne akan guntu na musamman tare da yankin da aka keɓe inda aka sanya sassan nama.Wannan yanki na kama yana ƙunshe da ɓangarorin ɓangarorin, kowanne ya yi daidai da wani wuri na musamman a cikin nama.Kwayoyin RNA da aka kama daga nama ana yi musu lakabi da kebantattun abubuwan gano kwayoyin halitta (UMIs) yayin aiwatar da rubutun baya.Waɗannan tabobin da aka ƙera da UMI suna ba da damar taswirar taswirar sararin samaniya da ƙididdige yawan magana a cikin ƙudurin tantai ɗaya.Haɗuwa da samfuran barcoded na sararin samaniya da UMI suna tabbatar da daidaito da ƙayyadaddun bayanan da aka samar.Ta amfani da wannan fasaha ta Spatial Transcriptomics, masu bincike za su iya samun zurfin fahimta game da tsarin sararin samaniya na sel da hadaddun hulɗar kwayoyin da ke faruwa a cikin kyallen takarda, suna ba da haske mai mahimmanci game da hanyoyin da ke tattare da tsarin ilimin halitta a fannoni da yawa, ciki har da oncology, neuroscience, ilmin halitta na ci gaba, rigakafi. , da kuma nazarin halittu.
Platform: 10X Genomics Visium da Illumina NovaSeq
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Cikakkun Tsawon mRNA Sequencing-Nanopore
Yayin da tsarin tsarin mRNA na tushen NGS ke aiki azaman kayan aiki iri-iri don ƙididdige maganganun kwayoyin halitta, dogaro da gajeriyar karantawa yana taƙaita tasirin sa a cikin hadaddun nazarin kwafin rubutu.Nanopore sequencing, a gefe guda, yana amfani da fasahar dogon karantawa, yana ba da damar jeri cikakken kwafin mRNA.Wannan tsarin yana sauƙaƙe bincike mai zurfi na madadin splicing, gene fusions, poly-adenylation, da ƙididdige abubuwan mRNA.
Jerin Nanopore ya dogara da siginonin lantarki na nanopore guda ɗaya na ainihin lokacin.Jagoran sunadaran motoci, DNA mai ɗaure biyu yana ɗaure zuwa sunadaran nanopore da aka saka a cikin biofilm, buɗewa yayin da yake wucewa ta tashar nanopore a ƙarƙashin bambancin ƙarfin lantarki.Ana gano siginonin lantarki na musamman waɗanda aka samar ta tushe daban-daban akan madaidaicin DNA kuma ana rarraba su a cikin ainihin lokaci, suna sauƙaƙe daidaitaccen jerin abubuwan nucleotide.Wannan sabuwar dabarar ta shawo kan iyakoki na ɗan gajeren karatu kuma tana ba da dandamali mai ƙarfi don ƙididdige ƙididdigar genomic, ya haɗa da hadaddun nazarin kwafi.
Platform: Nanopore Promethion P48
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Tsarin mRNA mai cikakken tsayi -PacBio
Yayin da tsarin mRNA na tushen NGS kayan aiki ne mai dacewa don ƙididdige maganganun kwayoyin halitta, dogaro da gajeriyar karantawa yana hana amfani da shi a cikin hadaddun nazarin kwafin rubutu.Sequencing na PacBio (Iso-Seq), a gefe guda, yana amfani da fasahar dogon karantawa, yana ba da damar jeri cikakken kwafin mRNA.Wannan tsarin yana sauƙaƙe bincike mai zurfi na madadin splicing, gene fusions da poly-adenylation ko da yake ba shine zaɓi na farko don ƙididdige maganganun kwayoyin halitta ba, saboda yawan adadin bayanai da ake buƙata.
Fasahar jeri ta PacBio ta dogara ne akan jerin kwayoyin halitta guda ɗaya, ainihin-lokaci (SMRT), suna ba da fa'ida ta musamman wajen ɗaukar kwafin mRNA mai cikakken tsayi.Wannan sabuwar dabarar ta ƙunshi amfani da sifili-yanayin waveguides (ZMWs), rijiyoyin microfabricated waɗanda ke ba da damar lura da ainihin ayyukan DNA polymerase yayin jerin gwano.A cikin waɗannan ZMWs, PacBio's DNA polymerase yana haɗa nau'in DNA na haɗin gwiwa, yana samar da dogon karatu wanda ya kai gabaɗayan kwafin mRNA.Aiki na PacBio a cikin yanayin Ijma'i na madauwari (CCS) yana haɓaka daidaito ta hanyar maimaita nau'ikan kwayoyin halitta iri ɗaya.Karatun HiFi da aka ƙirƙira yana da daidaito kwatankwacin NGS, yana ƙara ba da gudummawa ga ingantaccen ingantaccen bincike na fasalulluka masu rikitarwa.Platform: PacBio Sequel II
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Eukaryotic mRNA Sequencing-Illumina
Tsarin mRNA yana ba da ikon cikakken bayanin duk bayanan mRNA a cikin sel a ƙarƙashin takamaiman yanayi.Wannan fasaha mai yanke-yanke tana aiki azaman kayan aiki mai ƙarfi, yana buɗe bayanan bayanan ƙwayoyin cuta masu rikitarwa, tsarin halittar halitta, da hanyoyin ƙwayoyin ƙwayoyin cuta masu alaƙa da nau'ikan hanyoyin nazarin halittu.An karɓe shi sosai a cikin bincike na asali, bincike na asibiti, da haɓaka magunguna, jerin mRNA yana ba da haske game da rikitattun kuzarin salon salula da ka'idojin kwayoyin halitta.
Platform: Illumina NovaSeq X
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Matsakaicin tushen mRNA Sequencing-Illumina
Tsarin mRNA yana ba da ikon cikakken bayanin duk bayanan mRNA a cikin sel a ƙarƙashin takamaiman yanayi.Wannan fasaha mai yanke-yanke tana aiki azaman kayan aiki mai ƙarfi, yana buɗe bayanan bayanan ƙwayoyin cuta masu rikitarwa, tsarin halittar halitta, da hanyoyin ƙwayoyin ƙwayoyin cuta masu alaƙa da nau'ikan hanyoyin nazarin halittu.An karɓe shi sosai a cikin bincike na asali, bincike na asibiti, da haɓaka magunguna, jerin mRNA yana ba da haske game da rikitattun kuzarin salon salula da ka'idojin kwayoyin halitta.
Platform: Illumina NovaSeq X
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Dogon jerin abubuwan da ba codeing-Ilumina
Dogayen RNAs marasa coding (lncRNAs) RNA sun fi tsayi fiye da nucleotides 200 waɗanda ke da ƙaramin yuwuwar coding kuma abubuwa ne masu mahimmanci a cikin RNA marasa coding.An samo su a cikin tsakiya da cytoplasm, waɗannan RNAs suna taka muhimmiyar rawa a cikin epigenetic, transcriptional, and post-transcriptional ka'idojin, suna nuna mahimmancin su wajen tsara tsarin salula da kwayoyin halitta.Tsarin LncRNA kayan aiki ne mai ƙarfi a cikin bambance-bambancen Cell, Ontogenesis, da cututtukan ɗan adam.
Platform: Illumina NovaSeq
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Ƙananan jerin RNA-Illumina
Ƙananan kwayoyin RNA (sRNA), yawanci ƙarƙashin 200 nucleotides a tsayi, sun haɗa da microRNAs (miRNAs), ƙananan RNAs (siRNAs), da RNAs masu hulɗar piwi (piRNAs).Daga cikin waɗannan, miRNAs, kusan 20-24 nucleotides tsayi, sun shahara musamman don mahimman ayyukansu na tsari a cikin matakai daban-daban na salon salula.Tare da ƙayyadaddun tsarin nama da ƙayyadaddun yanayin magana, miRNAs suna nuna babban kiyayewa a cikin nau'ikan nau'ikan daban-daban.
Platform: Illumina NovaSeq
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circRNA sequencing-Ilumina
Tsarin RNA madauwari (circRNA-seq) shine yin bayanin martaba da nazarin RNAs madauwari, aji na kwayoyin RNA waɗanda ke haifar da rufaffiyar madaukai saboda abubuwan da ba na canonical ba, suna samar da waɗannan RNA tare da ƙarin kwanciyar hankali.Yayin da aka nuna wasu circRNAs suna aiki azaman soso na microRNA, suna sarrafa microRNAs da hana su daidaita mRNAs ɗin da suke son cimmawa, wasu circRNAs na iya yin hulɗa tare da sunadaran, daidaita maganganun kwayoyin halitta, ko suna da matsayi a cikin tsarin salula.Binciken furuci na circRNA yana ba da haske game da ayyukan gudanarwa na waɗannan ƙwayoyin cuta da mahimmancin su a cikin matakai daban-daban na salon salula, matakan haɓakawa, da yanayin cututtuka, yana ba da gudummawa ga zurfin fahimtar sarƙar ƙa'idar RNA a cikin mahallin maganganun kwayoyin halitta.
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Cikakkun jerin bayanan rubutu - Illumina
Gabaɗayan jeri na rubutun yana ba da cikakkiyar hanya don bayyana ƙwayoyin RNA daban-daban, wanda ya ƙunshi duka coding (mRNA) da RNAs marasa coding (lncRNA, circRNA, da miRNA).Wannan dabarar tana ɗaukar cikakken kwafin takamaiman ƙwayoyin sel a wani ɗan lokaci, yana ba da damar cikakkiyar fahimtar hanyoyin salula.Hakanan an san shi da "Jimlar jeri na RNA," yana da nufin buɗe rikitattun hanyoyin sadarwa na tsari a matakin rubutu, yana ba da damar zurfafa bincike kamar gasa RNA endogenous (ceRNA) da kuma nazarin RNA na haɗin gwiwa.Wannan yana nuna matakin farko zuwa ga halayen aiki, musamman a buɗe hanyoyin sadarwar da suka shafi hulɗar ceRNA-miRNA-mRNA tushen ceRNA.
