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PacBio-Full-tsawon 16S/18S/ITS Tsarin Amplicon
Amplicon (16S / 18S / ITS) dandamali an haɓaka shi tare da shekaru na gwaninta a cikin nazarin ayyukan bambance-bambancen microbial, wanda ya ƙunshi daidaitaccen bincike na asali da bincike na keɓaɓɓu: bincike na asali ya ƙunshi babban abun ciki na bincike na bincike na microbial na yanzu, abun ciki na bincike yana da wadata kuma cikakke. kuma ana gabatar da sakamakon bincike ta hanyar rahotannin aikin;Abubuwan da ke cikin keɓaɓɓen bincike sun bambanta.Za'a iya zaɓar samfurori kuma ana iya saita sigogi cikin sassauƙa bisa ga ainihin rahoton bincike da manufar bincike, don gane keɓaɓɓen buƙatun.Tsarin aiki na Windows, mai sauƙi da sauri.
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Rubutun Rubutun-cikakken-PacBio (Ba Nasiha ba)
Ɗaukar bayanan jeri na Biosciences na Pacific (PacBio) Isoform a matsayin shigarwa, wannan App yana da ikon gano jerin cikakkun bayanai na rubutun (ba tare da taro ba).Ta hanyar yin taswirar cikakken tsayin daka akan kwayoyin halitta, za a iya inganta kwafin ta hanyar sanannun kwayoyin halitta, kwafi, yanki, da sauransu.Binciken haɗin gwiwa tare da bayanan bayanan kwafi na NGS yana ba da damar ƙarin cikakkun bayanai da ƙarin ƙididdige ƙididdigewa a cikin magana a matakin kwafi, wanda ya fi fa'ida fa'ida ta ƙasa daban-daban da bincike na aiki.
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Kayan aiki
BMKCloud shine babban dandamali na bioinformatic wanda ke ba da mafita guda ɗaya don shirye-shiryen genomic, wanda masu bincike suka amince da su a fage daban-daban ciki har da likitanci, aikin gona, muhalli, da sauransu. BMKCloud yana da nau'ikan kayan aikin bioinformatic da ake amfani da su akai-akai da suka haɗa da annotation gene, kayan aikin halitta na juyin halitta, ncRNA, sarrafa ingancin bayanai, taro, daidaitawa, hakar bayanai, maye gurbi, ƙididdiga, janareta na adadi, jeri. nazari, da dai sauransu.
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kananan RNA
Kananan RNAs nau'in gajeriyar RNA ce mara coding tare da matsakaita tsawon 18-30 nt, gami da miRNA, siRNA da piRNA.An ba da rahoton cewa waɗannan ƙananan RNAs suna da yawa a cikin matakai daban-daban na ilimin halitta kamar lalata mRNA, hana fassarar, samuwar heterochromatin, da dai sauransu. An yi amfani da bincike na ƙananan RNA a cikin binciken game da ci gaban dabba / shuka, cututtuka, ƙwayoyin cuta, da dai sauransu. Ƙananan RNA. dandali na bincike na jerin abubuwa ya ƙunshi daidaitattun ƙididdiga da haɓaka bayanai na ci gaba.A kan tushen bayanan RNA-seq, daidaitaccen bincike na iya samun gano miRNA da tsinkaya, tsinkayar kwayar halittar miRNA, annotation da nazarin magana.Binciken ci gaba yana ba da damar bincike na miRNA na musamman da haɓakawa, tsarar zane na Venn, miRNA da ginin cibiyar sadarwa na manufa.
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NGS-WGS (Illumina/BGI)
NGS-WGS wani dandamali ne na sake-sake tsarin nazarin kwayoyin halitta, wanda aka haɓaka bisa tushen ƙwarewa a cikin Fasahar Biomarker.Wannan dandamali mai sauƙi-da-amfani yana ba da damar ƙaddamar da sauri na haɗaɗɗiyar aikin bincike ta hanyar saita ƴan sigar asali, waɗanda suka dace da bayanan jeri na DNA da aka samar daga dandamalin Illumina da dandamali na BGI.Ana tura wannan dandali akan uwar garken kwamfuta mai girma, wanda ke ba da ikon nazarin bayanai masu inganci a cikin ƙayyadaddun lokaci.Ana samun haƙar ma'adinan da aka keɓance bisa ƙa'idar bincike, gami da tambayar maye gurbi, ƙirar ƙirar PCR, da sauransu.
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mRNA(Reference)
Transcriptome shine hanyar haɗin gwiwa tsakanin bayanan kwayoyin halitta da kuma kariya ta aikin nazarin halittu.Ƙa'idar matakin rubutu ita ce mafi mahimmanci kuma mafi yawan binciken yanayin ƙa'idodin kwayoyin halitta.Tsarin kwafi na iya jera rubutun a kowane lokaci a cikin lokaci ko ƙarƙashin kowane yanayi, tare da ƙuduri daidai zuwa nucleotide guda ɗaya. Zai iya nuna ƙarfi sosai kan matakin kwafin kwayoyin halitta, a lokaci guda gano da ƙididdige rubuce-rubucen da ba kasafai da na al'ada ba, da samar da bayanan tsarin samfurin takamaiman kwafi.
A halin yanzu, an yi amfani da fasaha mai mahimmanci ta hanyar fasaha a fannin aikin gona, likitanci da sauran fannonin bincike, ciki har da ka'idojin ci gaban dabba da shuka, daidaita yanayin muhalli, hulɗar rigakafi, ganowar kwayoyin halitta, juyin halittar jinsin halittu da ƙari da gano cututtukan kwayoyin halitta.
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Metagenomics (NGS)
An tsara wannan dandalin bincike don nazarin bayanan metagenomic na harbi bisa tushen gogewar shekaru.Ya ƙunshi haɗaɗɗen aikin aiki wanda ya ƙunshi nau'ikan nazarin metagenomics da ake buƙata da yawa waɗanda suka haɗa da sarrafa bayanai, nazarin matakin nau'in nau'in, nazarin matakin matakin jinsi, metagenome binning, da sauransu. Bugu da ƙari, ana samun kayan aikin haƙar ma'adinai na musamman akan daidaitaccen aikin bincike, gami da binciken kwayoyin halitta da nau'in tambaya. , saitin siga, ƙirƙira adadi na musamman, da sauransu.
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LncRNA
Dogayen RNAs marasa coding (lncRNA) nau'in rubuce-rubuce ne masu tsayi sama da 200 nt, waɗanda ba sa iya ƙididdige sunadaran.Shaidar tarawa ta nuna cewa yawancin lncRNAs suna da yuwuwar yin aiki.Fasahar jeri mai girma da kayan aikin bincike na bioinformatic suna ba mu ikon bayyana jerin lncRNA da sanya bayanai cikin inganci kuma suna jagorantar mu don gano lncRNAs tare da mahimman ayyuka na tsari.BMKCloud yana alfahari da samar da dandamalin bincike na lncRNA na abokan cinikinmu don cimma saurin bincike, abin dogaro da sassauƙa na lncRNA.
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GWAS
Nazarin ƙungiyar genome-wide (GWAS) yana nufin gano bambance-bambancen kwayoyin halitta (genotype) waɗanda ke da alaƙa da takamaiman halaye (phenotype).Nazarin GWA yana binciken alamomin kwayoyin halitta sun haye dukkanin kwayoyin halittar mutane masu yawa kuma suna tsinkayar ƙungiyoyin genotype-phenotype ta ƙididdigar ƙididdiga a matakin yawan jama'a.Ci gaba dayan kwayoyin halitta na iya yuwuwar gano duk bambance-bambancen kwayoyin halitta.Haɗe-haɗe tare da bayanan phenotypic, GWAS za a iya sarrafa shi don gano phenotype SNPs, QTLs da ɗan takara genes, wanda karfi da goyon bayan zamani dabba / shuka kiwo.SLAF dabara ce da ta ɓullo da kai mai sauƙaƙan tsarin tsarin kwayoyin halitta, wanda ke gano alamomin rarrabawar kwayoyin halitta, SNP.Wadannan SNPs, a matsayin alamomin kwayoyin halitta, ana iya sarrafa su don nazarin haɗin gwiwa tare da halayen da aka yi niyya.Dabaru ce mai tsadar gaske wajen gano hadaddun halaye masu alaƙa da bambancin kwayoyin halitta.
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Nanopore Cikakken-tsawon kwafi
Matsakaicin madaidaicin madaidaicin isoforms a cikin kwayoyin halitta sune mahimman hanyoyin kwayoyin halitta don daidaita maganganun kwayoyin halitta da bambancin furotin.Mahimman ganewar sifofin rubutun shine tushen bincike mai zurfi na tsarin ka'idojin maganganun kwayoyin halitta.Dandali na jerin jerin Nanopore ya sami nasarar kawo nazarin rubutu zuwa matakin isoform.An tsara wannan dandalin bincike don yin nazarin bayanan RNA-Seq da aka samar akan dandalin Nanopore akan tushen tushen kwayoyin halitta, wanda ke samun ƙididdiga na ƙididdiga da ƙididdiga a duka matakin kwayoyin halitta da matakin rubutun.
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cir-RNA
Madauwari RNA (circRNA) wani nau'in RNA ne mara coding, wanda kwanan nan aka same shi yana taka muhimmiyar rawa a cikin hanyoyin sadarwar da ke da hannu wajen haɓakawa, juriyar muhalli, da sauransu. Ya bambanta da kwayoyin RNA na layi, misali mRNA, lncRNA, 3′ da 5′ An haɗa ƙarshen circRNA tare don samar da tsarin madauwari, wanda ya cece su daga narkewar exonuclease kuma sun fi kwanciyar hankali fiye da yawancin RNA na layi.An gano CircRNA yana da ayyuka daban-daban wajen daidaita maganganun kwayoyin halitta.CircRNA na iya yin aiki azaman ceRNA, wanda ke ɗaure miRNA gasa, wanda aka sani da soso miRNA.Dandalin bincike na circRNA yana ba da ikon tsarin circRNA da nazarin magana, hasashen manufa da nazarin haɗin gwiwa tare da sauran nau'ikan kwayoyin RNA.
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BSA
Dandali mai girma Segregant Analysis yana ƙunshe da daidaitaccen bincike na mataki ɗaya da bincike na gaba tare da saitin siga na musamman.BSA wata dabara ce da ake amfani da ita don gano alamomin kwayoyin halitta masu alaƙa da sauri.Babban tsarin aiki na BSA ya ƙunshi: 1. zabar ƙungiyoyi biyu na mutane waɗanda ke da ƙima sosai;2. hada DNA, RNA ko SLAF-seq (Biomarker ya Haɓaka) na kowane ɗaiɗai don samar da nau'in DNA guda biyu;3. gano jerin bambance-bambancen da aka saba da kwayar halitta ko tsakanin, 4. tsinkaya dan takarar da aka haɗa yankuna ta ED da SNP-index algorithm;5. Binciken aiki da haɓakawa akan kwayoyin halitta a yankunan yan takara, da dai sauransu. Ƙarin ma'adinai na ci gaba a cikin bayanai ciki har da gwajin alamar kwayoyin halitta da kuma zane-zane.
