Tsarin Tsiro/Dabbobi Gabaɗayan Halitta

Tsarin Tsiro/Dabbobi Gabaɗayan Halin Halittar Halittar Dabbobi da Aka Fitar da Hoton

Dukkanin tsarin sake-sake-tsarin kwayoyin halitta, wanda kuma aka sani da WGS, yana ba da damar bayyanar da sauye-sauye na yau da kullun da na yau da kullun akan dukkan kwayoyin halittar da suka hada da Single Nucleotide Polymorphism (SNP), Sharewa Shiga (InDel), Bambancin Tsarin (SV), da Bambancin Lamba Kwafi (CNV). ).SVs sun ƙunshi babban ɓangare na tushen bambancin fiye da SNPs kuma suna da tasiri mafi girma akan kwayoyin halitta, wanda ke da tasiri mai mahimmanci akan rayayyun halittu.Tsawon karatu mai tsawo yana ba da damar ƙarin ainihin gano manyan gutsuttsura da bambance-bambance masu rikitarwa saboda dogon karantawa yana ba da sauƙin hayewar chromosomal akan yankuna masu rikitarwa kamar maimaita tandem, yankuna masu wadatar GC/AT, da yankuna masu saurin canzawa.

Platform: Illumina, PacBio, Nanopore

Tuntube Mu

Cikakkun Sabis

Sakamakon Demo

Fitar Bugawa

1. Amfanin Sabis

Ƙwarewa mai zurfi a cikin jerin kwayoyin halitta don fiye da nau'in 1000.

Fiye da shari'o'i 800 da aka buga tare da tarin tasirin tasirin sama da 4000.

Cikakken bincike na bioinformatics akan bambancin kira da nazarin ayyuka.

2. Bayanin Sabis

Dandalin		Laburare	Shawarar zurfin seq
Illumina		Farashin PE150	Don SNP, kiran InDel ≥ 10x Don SV, kiran CNY ≥ 30x
Nanopore		8 kb ku	Don SV, kiran CNY ≥ 20x
Pacbio	CCS	15 kb	Don kiran SNP, InDel, SV, CNY ≥ 10x

3. Samfuran Bukatun

Dandalin		Conc.(ng/μL)	Adadin (ng)	Tsafta		Agarose gel
				OD260/280	OD260/230	1. Share babban band tare da babu ko iyakalalata da aka lura akan gel. 2. Babu ko iyakance RNA ko gurɓataccen furotin
Illumina		≥1	≥30	-	-
Nanopore		≥30	Ya dogara da yawan amfanin bayanai 10 μg/ce	1.7-2.2	≥1.5
Pacbio	CCS	≥50		1.7-2.2	1.8-2.5

4. Binciken Halittu

5. Gudun Aikin Sabis

Samfurin bayarwa

Cire DNA

Gina ɗakin karatu

Jeri

Binciken bayanai

Isar da bayanai

Na baya: BMKMANU S1000 Spatial Transcriptome

Na gaba: Single-nucleus RNA Sequencing

1) Kididdigar Taswirar Genome

Table 1 Kididdigar sakamakon taswira

Hoto 1 Rarraba girman saka da karanta ɗaukar hoto.

2) Bambance-bambancen Ganewa

Hoto 2 Kididdigar da bayanin SNP/INDEL/SV tsakanin Samfura

Hoto 3 Genom-fadi rarraba vatiations

3) Bayanin aiki na bambancin

2019	Sadarwar yanayi	Gabaɗaya-genome mai kama da ita yana bayyana asalin Brassica napus da yanayin ƙwayoyin halittar da ke cikin haɓakarsa
2020	PNAS	Asalin juyin halitta da tarihin gida na kifin zinare (Carassius auratus)
2021	Jaridar Biotechnology Journal	Bayanin kwayoyin halittar nau'in jute guda biyu da aka noma
2021	Jaridar Biotechnology Journal	Sa hannu na genomic kayan lambu da ƙwayar mai allopolyploid Brassicajuncea da loci na kwayoyin halitta wanda ke sarrafa tarin glucosinolates.
2019	Shuka Kwayoyin Halitta	Gabaɗaya-genome mai kama da tarin nau'ikan nau'ikan fyade a duniya yana bayyana tushen asalin halittar halittarsu
2022	Binciken Horticulture	Binciken haɗin gwiwar genome-fadi yana ba da fahimtar kwayoyin halitta game da bambancin yanayin girman irin kankana
2021	Jaridar Gwajin Botany	Nazarin ƙungiyar genome-fadi ya gano bambance-bambancen GhSAD1 da ke ba da juriyar sanyi a cikin auduga.
2021	Jaridar Gwajin Botany	Nazarin ƙungiyar genome-fadi da kwatancen kwatancen sun nuna sabon labari QTL da ƙwayoyin ɗan takara waɗanda ke sarrafa girman fure a cikin nau'in fyade.