JUYIN HALITTA
Genome De novo
Taro
Abstract
"Alamar alama ta seadragons ta haɗa da abubuwan da ke kama da ganye, bakin tubular haƙori, da ciki na maza wanda ya haɗa da shirya ƙwai da aka haɗe akan buɗaɗɗen “patch”.Mu de novo – jerin kwayoyin halittar maza da mata na ruwan teku na gama-gari (Phyllopteryx taeniolatus) da nau’in da ke da alaƙa da shi, da alligator pipefish (Syngnathoides biculeatus).Bayanan bayanan rubutu daga sabon salo na juyin halitta, abubuwan da ke kama da ganye, sun nuna cewa an haɗa nau'ikan kwayoyin halitta waɗanda galibi ke da hannu wajen haɓaka fin tare da haɓakar rubutun don yuwuwar gyara nama da ƙwayoyin kariya na rigakafi.Mutantan zebrafish na scpp5, wanda ya ɓace a cikin duk syngnathids, an gano cewa ba shi da ko kuma ya lalata haƙoran pharyngeal, yana goyan bayan ra'ayin cewa asarar scpp5 ya haifar da asarar hakora a syngnathids.Jima'i mai sanyawa-ƙayyade wurin da ke ɓoye wani takamaiman jinsin amhr2y na namiji wanda aka raba ta ruwan teku da alligator pipefish. ”
Kaya da matakai:
Kayayyaki
Fruwan tekun mu gama gari (P. taeniolatusda pipefish guda biyu (alligator pipefish)S. biculeatus) samfurori.An yi amfani da magudanar ruwa guda biyu (namiji ɗaya da mace ɗaya) da pipefish guda biyu (namiji ɗaya da mace ɗaya) waɗanda aka yi amfani da su don tsarin tsarin halittar gabaɗaya, kuma an yi amfani da wasu maza biyu na kowa na teku don yin kamannin kwayoyin halitta.
Dabarun Sequencing na Genome
270/350 bp library (Hiseq 2500) + Pacbio 20Kb (Sequel) ko Nanopore 30 Kb (MinION)+ Hi-C (NovaSeq 6000) .sequencing, da kuma wasu maza biyu na kowa seadragon mutane da aka yi amfani da genome resequencing.
Tsarin Rubutu
Fko na kowa seadragon, kwakwalwa, ido, gill, hanta, zuciya, koda, mesentery, hanji, tsoka, fin, fata, ganye-kamar appendages, testis, da ovary aka tattara.
Tsarin taro na Genome de novo
Canu (Gyara) + WTDBG2 (Majalisa) + Pilon (Yaren mutanen Poland) + Lachesis (Hi-C).
Babban Sakamako
Hoto 1. Mahimman siffofi na ruwan teku na kowa (P. taeniolatus) da kuma alligator pipefish (S. biaculeatus) da matsayi na phylogenetic.
Hoto 2. Ilimin halittar jiki da kayan shafa na kwayoyin halitta na kayan lambu masu kama da ganye a cikin tekun na kowa (P. taeniolatus).
Hoto 3. Ƙididdigar ƙayyadaddun jima'i a cikin ruwan teku na kowa (P. taeniolatus) da kuma alligator pipefish (S. biaculeatus).
Hoto 4. phenotypes na haƙorin pharyngeal a cikin zebrafish scpp5 homozygous mutants.
Lokacin aikawa: Janairu-08-2022