MICROBIAL
Daidaitawar ƙwayoyin cuta da rashin motsi tare da haɓakar haɓakar tebuconazole, abun da ke cikin ƙasa microbiome da aiki.
Tsarin amplicon mai cikakken tsayi 16S |PacBio HiFi |Bambancin Alpha |Bambancin beta
A cikin wannan binciken, cikakken tsawon 16S amplicon sequencing ta PacBio da bioinformatic analysis an bayar da su ta Biomarker Technologies.
Karin bayanai
Biochar immobilized tebuconazole-degrading bakteriya Alcaligenes faecalis WZ-2 an yi nazari akan ingancin biodegradation kuma yana tasiri akan gurbataccen ƙasa na tebuconazole idan aka kwatanta da nau'in wulakanci kyauta WZ-2.
1. Biochar-immobilized WZ-2 ya nuna raguwa mai inganci a cikin tebuconazole idan aka kwatanta da WZ-2 kyauta ta hanyar rage tebuconazole rabin rayuwa a cikin ƙasa daga kwanaki 18.7 zuwa kwanaki 13.3.
2. Biochar-immobilized WZ-2 sun sami damar mayar da ƙasa na ƙasa microbial enzyme ayyuka, ciki har da urease, dehydrogenase da invertase, da dai sauransu.
3. Bayanan ƙananan ƙwayoyin cuta a cikin biochar-immobilized WZ-2 da aka kula da ƙasa da aka ƙaddara ta hanyar cikakken tsayin 16S jerin suna da goyon baya mai ƙarfi cewa wannan tsarin zai iya dawo da lafiyar ƙasa ta hanyar inganta tsarin al'umma na kwayan cuta a ƙarƙashin gurɓataccen tebuconazole.
Gwaji (mai alaƙa da jerin abubuwa)
Ƙungiya: CK: Ƙasar halitta;T: Ƙasa spiked tare da tebuconazole;S: Tebuconazole ƙunshi ƙasa tare da free iri WZ-2;BC: Tebuconazole ya ƙunshi ƙasa tare da biochar;BCS: Tebuconazole ya ƙunshi ƙasa tare da biochar immobilized WZ-2.
Samfura: Jimillar hakar DNA na ƙasa wanda aka haɓaka ta 16S rDNA na farko
27F (5'-AGAGTTTGATCCTGCTCAG-3') da 1492R (5'-GGTTACCTTGTTACCA),Maimaita cikakken tsawon 16S rDNA
Tsarin tsari: PacBio RS II
Dabarun jeri: CCS HIFI yana karantawa
Binciken bayanai:BMKCloudBioinformatic Platforming yana sa kifin zinare ya zama kyakkyawan tsarin ƙirar halitta don ilimin halittar kifin da juyin halitta.
Sakamako
Tsarin al'umma na ƙananan ƙananan ƙasa an ƙaddara ta hanyar 16S rDNA.wadatar OTU da ƙididdigar alpha iri-iri, gami da Chao1, Ace, Shannon da Simpson an kimanta su don bayyana bambancin nau'in kowane tsari.Bayan shafe kwanaki 60, duk fihirisa sun nuna irin wannan yanayin, watau tebuconazole na iya haifar da raguwar wadatar jinsuna da bambancin ƙasa.Koyaya, ta hanyar ƙara nau'in WZ-2, al'ummar ƙwayoyin cuta na ƙasa an dawo da wani ɗan lokaci dangane da wadatuwa da iri iri.An lura da iyakanceccen bambanci tsakanin BC, BCS da CK, yana nuna cewa biochar da biochar-immobilized WZ-2 na iya dawo da lafiyar halittun ƙasa yadda ya kamata.
An yi amfani da Hanyar Ƙungiya Biyu mara nauyi tare da Ma'anar Arithmetic (UPGMA) a cikin wannan binciken don nuna bambancin beta tsakanin ƙungiyoyi.Kamar yadda aka nuna a cikin adadi mai zuwa, BC, BSC da CK sun raba irin wannan tsarin na ƙwayoyin cuta idan aka kwatanta da rukunin T da S, wanda ya ƙara nuna cewa gabatarwar biochar a cikin gurɓataccen ƙwayar ƙasa na tebuconazole zai iya sauƙaƙa dawo da ƙananan ƙwayoyin cuta a cikin ƙasa.
Hoto.Tarin UPGMA na al'ummar kwayan cuta a matakin phylum ƙarƙashin jiyya daban-daban
Magana
Sun, Tong, et al."Dacewar kwayoyin cuta da rashin motsa jiki tare da haɓakar haɓakar tebuconazole, abun da ke cikin ƙasa microbiome da aiki."Jaridar Abubuwa masu haɗari398 (2020): 122941.
Labarai da Labarai yana da niyyar raba sabbin maganganu masu nasara tare da Biomarker Technologies, ɗaukar sabbin nasarorin kimiyya gami da fitattun dabarun da aka yi amfani da su yayin binciken.
Lokacin aikawa: Janairu-08-2022