GWAS
Take: Cikakken-genome mai kama da shi yana bayyana asalin Brassica napus da loci na kwayoyin da ke cikin haɓakarsa.
Jarida: Sadarwar yanayi
NGS |WGS |Maimaitawa |GWAS |Rubutu |RNAseq |Brassica napus |Juyin Halitta |Zaman gida
A cikin wannan binciken, Biomarker Technologies ya ba da Sabis akan jerin abubuwan NGS, da kuma goyon bayan fasaha akan nazarin bioinformatics akan bayanan jeri.
Fage
Brassica napus(fyade) muhimmin amfanin gona ne na iri mai kuma kyakkyawan samfuri don bincika hanyoyin bincike na ƙayyadaddun polyploid, juyin halitta da zaɓi.Duk da haka, ko jinsunan daji ko masu ba da gudummawa na gida sun kasance kakannin iyaye da kuma kwayoyin halitta waɗanda suka ba da gudummawa ga gida da inganta ƙwayar fyade har yanzu ba a sani ba.
kaya da matakai
Kayayyaki:588B. nafisaAn shiga cikin wannan binciken, ciki har da 466 daga Asiya, 102 daga Turai, 13 daga Arewacin Amirka, da 7 daga Australia.Dangane da bayanan al'ada na girma, an raba waɗannan kayan zuwa nau'ikan halittu guda uku;spring (86 accessions), hunturu (74 accessions), da kuma Semi-hunturu (428 accessions).
Jeri:Matsakaicin kusan5× (jere daga 3.37× zuwa 7.71×)
Dandalin jerin abubuwa:Illumina Hisek 4000
Samar da bayanai:4.03 Tb tsaftataccen bayanai
Kira na SNP:BWA + GATK.5,294,158 SNPs da 1,307,151 InDels an samu.
Sakamako
Asalin B. napus
B. nafisaSubgenome ya samo asali ne daga kakan turnip na Turai.Lamarin kwararar kwayoyin halitta daga turnip Turawa zuwaB. napuSubgenome ya faru ~106-1170 shekaru da suka wuce.B. nafisaC subgenome mai yiwuwa ya samo asali ne daga kakannin kakannin waɗannan zuriyar.KakanB. nafisarabu daga kakannin kowa na nau'ikan nau'ikan nau'ikan B. oleracea guda huɗu, tare da kwararar ƙwayoyin halittar kwanan nan zuwa cikinB. nafisa~ 108-898 shekaru da suka wuce.B. nafisaC subgenome yana da asali mai rikitarwa fiye da A Subgenome.Babban Belltneck mai ƙarfi ya ci gaba a cikin duka ɓangarorin subgenomes biyu a lokacinB. nafisajuyin halitta.Da hunturu da kuma rabin-hunturuB. nafisaecotypes sun bambanta ~ 60 shekaru da suka wuce, yayin da hunturu da bazaraB. nafisaya bambanta ~ 416 da suka wuce, da iri mai da maras maiB. nafisaya bambanta ~ 277 shekaru da suka wuce.
Hoto 2 Tsarin yawan jama'a na 588 B. napus accessions da 199 na B. rapa accessions.
Hoto 3 Tsarin yawan jama'a na 588 B. napus accessions da 119 na B. oleracea accessions
Alamomin zaɓi da kuma nazarin ƙungiyar Genome-fadi.
A lokacin mataki na farko na ingantawa (FSI), an sami ƙarin bambance-bambancen kwayoyin halitta a cikin subgenome na B. napus C fiye da na A subgenome.Ƙananan bambance-bambancen kwayoyin halitta sun faru a lokacin FSI fiye da lokacin mataki na biyu na ingantawa (SSI).Genes a cikin SSI-zaɓin siginar siginar sun wadatar da su cikin jurewar damuwa, haɓakawa da hanyoyin rayuwa.60 loci da ke da alaƙa da halaye 10 da aka yi niyya, gami da 5 masu alaƙa da yawan iri, 3 zuwa tsayin siliki, 4 zuwa abun cikin mai, da 48 zuwa ingancin iri an gano.
Hoto 4 Binciken-fadi na Genome da bayanai na yankuna da aka zaɓa yayin SSI na B. napus
Binciken kwafi
Bayanan RNAseq na kyallen takarda na 11 daga babban abun ciki mai-mai da nau'i-nau'i-nau'i-nau'i-nau'i-nau'i-nau'i-nau'i da nau'i-nau'i-nau'i-nau'i biyu da aka gano kwayoyin halittar da ke da alaƙa da tsarin biosynthetic glucosinolate sun kasance da yawa fiye da wakilci.
Hoto 5 Bayanin ƙa'idodin lokacin furanni a ƙarƙashin zaɓin haɓakar yanayin halitta na B. napus
Tattaunawa
Wannan binciken ya ba da hanya mai mahimmanci don fahimtar asali da inganta tarihinB. nafisakuma zai sauƙaƙa rarraba tushen tushen halittar agronomic hadaddun halaye masu mahimmanci.Mahimmancin SNPs da ke da alaƙa da bambance-bambance masu kyau, siginar zaɓi da ƙwayoyin ɗan takara za su ba da gudummawa sosai a nan gaba, musamman don haɓaka yawan amfanin ƙasa, ingancin iri, abun cikin mai, da daidaitawar wannan amfanin gona na allopolyploid na kwanan nan da danginsa.
Magana
Gabaɗaya-genome mai kama da juna yana bayyana asalin Brassica napus da yanayin ƙwayoyin halittar da ke cikin haɓakawa[J].Sadarwar yanayi, 2019, 10 (1).
Labarai da Labarai yana da niyyar raba sabbin maganganu masu nasara tare da Biomarker Technologies, ɗaukar sabbin nasarorin kimiyya gami da fitattun dabarun da aka yi amfani da su yayin binciken.
Lokacin aikawa: Janairu-05-2022