JUYIN HALITTA
Kwatancen kwatankwacin kwayar halitta yana nuna haɓakar genome mai tsaka-tsakin tsaka-tsaki da kuma tsarin gine-ginen juyin halitta na folding 3D a cikin auduga.
Tsarin Nanopore |Hi-C |Tsarin PacBio |Illumina |RNA-sequencing |3D tsarin gine-gine na genome |Transposon |Kwatanta kwayoyin halitta
A cikin wannan binciken, Biomarker Technologies ya ba da goyon bayan fasaha akan jerin Nanopore, Hi-C da kuma nazarin halittu masu dacewa.
Abstract
An gane ƙarawa mai jujjuyawa (TE) a matsayin ƙarfin tuƙi mai yin sulhu da faɗaɗa girman kwayar halitta da juyin halitta, amma sakamakon ƙera gine-ginen genomic na 3D ya kasance ba a san shi sosai a cikin tsire-tsire ba.Anan, mun bayar da rahoton taro-girma na genomes don nau'ikan auduga guda uku masu ninki uku cikin girman kwayar halitta, wato.Gossypium rotundifolium(K2),G. arboreum(A2), kumaG. raimondii(D5), ta amfani da Oxford Nanopore Technologies.Kwatancen kwatankwacin genome yana tattara cikakkun bayanai na ƙayyadaddun ƙayyadaddun TE na layi wanda ke ba da gudummawa ga manyan bambance-bambancen girman kwayoyin halitta (K2, 2.44 Gb; A2, 1.62 Gb; D5, 750.19 Mb), kuma yana nuna ingantaccen abun ciki na kwayoyin halitta da alaƙar haɗin gwiwa tsakanin kwayoyin halitta.Mun gano cewa kusan 17% na kwayoyin halitta suna nuna canjin yanayin chromatin tsakanin sassan masu aiki ("A") da marasa aiki ("B"), kuma haɓakawar TE yana da alaƙa da haɓakar rabon A a cikin sassan jinsin (~ 7,000 genes). ) a cikin K2 da A2 dangane da D5.Kashi 42% na iyakoki masu haɗin kai (TAD) kawai an kiyaye su a tsakanin kwayoyin halitta guda uku.Bayananmu yana haifar da haɓaka TEs na baya-bayan nan bayan samar da takamaiman iyakokin TAD.Wannan binciken yana ba da haske game da rawar da ke tattare da yaduwar kwayar halitta ta transposon a cikin juyin halittar chromatin mafi girma a cikin tsirrai.
Mahimmin ƙididdiga na taro na genome
Hoto.Haɗin Genome da bayanin fasalin G. rotundifolium (K2)
Labarai da Labarai yana da niyyar raba sabbin maganganu masu nasara tare da Biomarker Technologies, ɗaukar sabbin nasarorin kimiyya gami da fitattun dabarun da aka yi amfani da su yayin binciken.
Lokacin aikawa: Janairu-05-2022