Tsarin Metagenomic-Nanopore
Amfanin Sabis
● Babban High-High-girma-Inganta daidaito na Sanarwar tantanin halitta da Hasashen Masana
● Rufewar keɓewar kwayoyin halittar ƙwayoyin cuta
● Ƙarin ƙarfi kuma abin dogaro a aikace a wurare daban-daban, misali gano ƙwayoyin cuta na ƙwayoyin cuta ko ƙwayoyin cuta masu alaƙa da ƙwayoyin cuta.
● Kwatanta metagenome bincike
Ƙayyadaddun Sabis
| Dandalin | Jeri | Bayanan da aka ba da shawarar | Lokacin Juya |
| Nanopore | ONT | 6 G/10 | 65 Kwanakin aiki |
Binciken bioinformatics
● Raw data ingancin kula
● Taron metagenome
● Saitin kwayoyin halitta da ba a sake sakewa ba
● Nazari iri-iri
● Binciken bambancin aikin kwayoyin halitta
● Bincike tsakanin ƙungiyoyi
● Binciken ƙungiyar akan abubuwan gwaji
Samfuran Bukatun da Bayarwa
Samfurin buƙatun da bayarwa
Samfuran Bukatun:
DominCire DNA:
| Nau'in Misali | Adadin | Hankali | Tsafta |
| Cire DNA | 1-1.5 g | 20 ng/ml | OD260/280= 1.6-2.5 |
Don samfuran muhalli:
| Nau'in samfurin | Hanyar samfurin da aka ba da shawarar |
| Ƙasa | Adadin samfur: kimanin.5 g;ku.Ya kamata a cire ragowar abin da ya bushe daga saman;Niƙa manyan guda kuma ku wuce ta 2 mm tace;Samfuran Aliquot a cikin bakararre EP-tube ko cyrotube don ajiya. |
| Najasa | Adadin samfur: kimanin.5 g;ku.Tattara da liquot samfurori a cikin bakararre EP-tube ko cryotube don ajiyar wuri. |
| Abubuwan ciki na hanji | Ana buƙatar sarrafa samfuran ƙarƙashin yanayin aseptic.Wanke nama da aka tattara tare da PBS;Centrifuge PBS kuma tattara hazo a cikin EP-tuben. |
| Lalacewa | Adadin samfur: kimanin.5 g;ku.Tattara da liquot samfurin sludge a cikin bakararre EP-tube ko cryotube don ajiya |
| Ruwan ruwa | Don samfurin tare da iyakataccen adadin ƙwayoyin cuta, kamar ruwan famfo, ruwan rijiya, da sauransu, Tattara ruwa aƙalla 1 L kuma ku wuce ta 0.22 μm tace don wadatar da ƙananan ƙwayoyin cuta akan membrane.Ajiye membrane a cikin bututu bakararre. |
| Fatar jiki | A hankali a goge saman fata tare da swab ɗin auduga mai bakararre ko ɓawon tiyata sannan a sanya shi cikin bututu mara kyau. |
Isar da Samfurin Nasiha
Daskare samfuran a cikin ruwa nitrogen na sa'o'i 3-4 kuma adana a cikin ruwa nitrogen ko -80 digiri zuwa ajiyar dogon lokaci.Ana buƙatar jigilar samfuri tare da bushe-kankara.
Gudun Aikin Sabis
Samfurin bayarwa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
1.Heatmap: Nau'in wadata tari
2.Ayyukan kwayoyin halitta annotate zuwa KEGG hanyoyin rayuwa
3.Species dangantaka cibiyar sadarwa
4.Circos na CARD kwayoyin juriya
BMK Case
Nanopore metagenomics yana ba da damar gano saurin ganewar asibiti na ƙwayoyin cuta na ƙananan ƙwayoyin cuta
Buga:Nature Biotechnology, 2019
Babban Halayen Fasaha
Tsarin: Nanopore MinION
Clinical metagenomics bioinformatics: Mai watsa shiri ragewar DNA, WIMP da ARMA bincike
Ganewar gaggawa: 6 hours
Babban hankali: 96.6%
Sakamako mai mahimmanci
A cikin 2006, ƙananan cututtukan numfashi (LR) ya haifar da mutuwar mutane miliyan 3 a duniya.Hanyar da aka saba don gano ƙwayoyin cuta na LR1 ita ce noma, wanda ke da rashin hankali, dogon lokaci-lokaci kuma rashin jagora a farkon maganin rigakafi.Gaggawa da sauri da daidaiton ƙwayoyin cuta ya daɗe yana buƙatar gaggawa.Dokta Justin daga Jami'ar Gabashin Anglia tare da abokan aikinsa sun yi nasarar ƙera hanyar Nanopore na metagenomic don gano ƙwayoyin cuta.Dangane da aikin su, 99.99% na DNA mai gida na iya raguwa.Ana iya gama gano ƙwayoyin cuta da ƙwayoyin ƙwayoyin cuta a cikin sa'o'i 6.
Magana
Charalampous, T. , Kay, GL , Richardson, H. , Aydin, A. , & O'Grady, J. .(2019).Nanopore metagenomics yana ba da damar gano saurin ganewar asibiti na ƙwayoyin cuta na ƙananan ƙwayoyin cuta.Halitta Biotechnology, 37(7), 1.
