Fungal Genome

Biomarker Technologies suna samar da binciken kwayoyin halitta, kyawawan kwayoyin halitta da kuma cikakkiyar kwayar halittar pene na fungal dangane da takamaiman manufar bincike.Za'a iya samun jerin abubuwan da ke tattare da kwayoyin halitta, taro da bayanin aiki ta hanyar haɗa jerin tsaran gaba + Tsarin tsara na uku don cimma babban taro na genome.Hakanan ana iya amfani da fasahar Hi-C don sauƙaƙe haɗuwar kwayoyin halitta a matakin chromosome.

Dandalin:PacBio Sequel II

Nanopore PromethION P48

Illumina NovaSeq Platform

Tuntube Mu

Cikakkun Sabis

Sakamakon Demo

Amfanin Sabis

● Dabarun jeri da yawa akwai don maƙasudin bincike daban-daban

● Ƙwarewa sosai a cikin taron kwayoyin halittar fungi tare da fiye da 10,000 na pene-complete genome da aka haɗa.

● Ƙwararrun goyon bayan fasaha na goyon bayan sana'a wanda ke cika ƙarin takamaiman bukatun bincike.

Ƙayyadaddun Sabis

Sabis	Dabarun Jeri	Garanti mai inganci	Kiyasin lokacin juyawa
Fungal lafiya taswira	Illumina 50X+ Nanopore 100X	Ci gaba N50≥2 Mb	35 kwanakin aiki
Fungal lafiya taswira	PacBio HiFi 30X	Ci gaba N50≥2 Mb	35 kwanakin aiki
Cikakkun taswirar pene na fungal	Illumina 50X+ Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	Matsakaicin anchoring na chromosome> 90%	45 kwanakin aiki

Binciken bioinformatics

● Raw data ingancin kula

● Taron al'ada

● Binciken bangaren kwayoyin halitta

● Bayanin aikin Halittu

● Kwatankwacin nazarin kwayoyin halitta

Samfuran Bukatun da Bayarwa

Samfuran Bukatun:

DominCire DNA:

Nau'in Misali	Adadin	Hankali	Tsafta
Cire DNA	1.2 μg	20 ng/ml	OD260/280= 1.6-2.5

Don samfuran nama:

Nau'in samfurin

Nasihar samfurin magani

Samfurin ajiya da jigilar kaya

Fungus Unicellular

Kula da yisti a ƙarƙashin microscope kuma tattara su a cikin lokacin ma'anar su

Canja wurin al'ada (wanda ke ɗauke da kusan sel 3-4.5e9) zuwa 1.5 ko 2 ml eppendorf.(Ci gaba akan kankara)

Centrifuge bututu na 1 min a 14000 g don tattara ƙwayoyin cuta kuma a cire abin da ke sama a hankali.

Rufe bututun kuma daskare ƙwayoyin cuta a cikin ruwa nitrogen na akalla sa'o'i 1-3.Ajiye bututu a cikin firiji -80 ℃.

Daskare samfuran a cikin ruwa nitrogen na sa'o'i 3-4 kuma adana a cikin ruwa nitrogen ko -80 digiri zuwa ajiyar dogon lokaci.Ana buƙatar jigilar samfuri tare da bushe-kankara.

Macro Fungus

Ana ba da shawarar nama a cikin lokacin girma sosai.

Kurkure nama tare da ruwan da ba shi da endotoxin, sannan 70% ethonal.

Ajiye samfurin a cikin bututun cryo.