Tsarin DNA/RNA - Mai bin Nanopore

Mabiyan DNA/RNA - Mabiyi Nanopore da Aka Fitar da Hoton

Sequencing na ONT wani nau'in kwayoyin halitta guda ɗaya ne na fasaha na siginar lantarki na ainihin lokacin bisa ga nanopores, ka'idar jerin kowane dandamali iri ɗaya ce.DNA/RNA mai ɗaure biyu zai ɗaure da sunadarin nanoporous wanda aka saka a cikin biofilm kuma yana buɗewa ƙarƙashin jagorancin furotin na motsa jiki, ƙarƙashin aikin bambancin ƙarfin lantarki daga ɓangarorin biofilm, sassan DNA / RNA suna wucewa ta hanyar furotin na tashar nanopore a wani takamaiman. ƙimar.Saboda bambance-bambancen abubuwan sinadarai na tushe daban-daban akan madaidaicin DNA/RNA, lokacin da tushe ɗaya ko kwayar DNA ta ratsa tashar nanopore, zai haifar da canjin siginar lantarki daban-daban.Ta hanyar ganowa da dacewa da waɗannan sigina, ana iya ƙididdige nau'ikan tushe daidai, kuma ana iya kammala gano ainihin lokacin.

Tuntube Mu

Cikakkun Sabis

Sakamakon Demo

Fasalolin Cikakkun Sabis

Dandalin	Girman Laburare	Abubuwan Haɓaka Bayanan Ƙirar (Kowace Tantalli)	Daidaiton tushe guda ɗaya	Aikace-aikace
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/cell	85-92%	Kiran SV, De novo, Cikakkun jeri, Iso-Seq, Bayanin Gene, Gano DNA methylation

Amfanin Sabis

● Kwarewar fiye da shekaru 5 akan dandamali na jerin abubuwan PacBio tare da dubban rufaffiyar ayyukan tare da nau'ikan nau'ikan iri daban-daban.
● BMKGENE abokin tarayya ne na Oxford Nanopore, tare da takaddun shaida na RNA/DNA.
● Akwai samfurori na yau da kullun na masu siye tare da cikakkun kayan aiki da isassun kayan aiki na jerin abubuwa.
● Dangane da dandalin Nanopore, an buga binciken binciken Denovo fiye da 10 dabbobi da shuka a cikin shahararrun mujallu na duniya.

Samfuran Bukatun

Nau'in Misali

Adadin

Ƙaddamarwa (Qubit ®)

Ƙarar

Tsafta

Wasu

Genomic DNA

Ya dogara da Bukatun Bayanai

≥20ng/μl

≥15 ml

OD260/280=1.7-2.2;

OD260/230≥1.5;

Share kololuwa a 260 nm, babu gurɓatawa

Ana buƙatar auna hankali ta hanyar Qubit da Qubit/Nanopore ≤ 2

Jimlar RNA

≥1.2μg

≥100μg/ml

≥15 ml

OD260/280=1.7-2.5;

OD260/230=0.5-2.5;babu gurbacewa

Darajar RIN ≥7.5

Gudun Aikin Sabis

Samfurin shiri

Gina ɗakin karatu

Jeri

Binciken bayanai

Isar da aikin

Na baya: Sequencing DNA/RNA - Illumina Sequencer

Na gaba: Takamaiman-Locus Amplified Sequencing Sequencing (SLAF-Seq)

Ƙididdigar Ingancin Bayanai na Samfurin DNA

Tebur 1. Kididdigar kan bayanai mai tsabta.

BMKID	rawSeqNum	rawSumBase	cleanSeqNum	CleanSumBase	N50 Len	CleanN90 Len	cleanMeanLen	cleanMaxLen	cleanMa'anaQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9