Kayayyaki

Fasahar sequencing na Illumina, dangane da Sequencing by Synthesis (SBS), ƙirƙira ce ta NGS ta duniya wacce ke da alhakin samar da sama da kashi 90% na bayanan jeri na duniya.Ka'idar SBS ta ƙunshi ɗaukar hoto mai lakabi mai jujjuyawa kamar yadda aka ƙara kowane dNTP, kuma daga baya an raba shi don ba da damar haɗa tushe na gaba.Tare da duk dNTPs guda huɗu masu jujjuyawa masu ɗaure da ke akwai a cikin kowane zagayowar zagayowar, gasa ta yanayi tana rage son haɗawa.Wannan fasaha mai jujjuyawar tana goyan bayan dakunan karatu guda-ɗaya da masu haɗa-zuwa-ƙarshe, suna ba da kewayon aikace-aikacen kwayoyin halitta.Illumina sequencing's high-throughput capabilities and madaidaicin sanya shi a matsayin ginshiƙi a cikin binciken kimiyyar halittu, ƙarfafa masana kimiyya don buɗe ɓarna na kwayoyin halitta tare da cikakkun bayanai da inganci.

DNBSEQ, wanda BGI ta haɓaka, wata sabuwar fasaha ce ta NGS wacce ta yi nasarar rage raguwar farashin jeri da haɓaka kayan aiki.Shirye-shiryen dakunan karatu na DNBSEQ ya ƙunshi rarrabuwar DNA, shirye-shiryen ssDNA da haɓaka da'ira don samun DNA nanoballs (DNB).Ana ɗora waɗannan a kan ƙaƙƙarfan wuri kuma daga baya ana yin su ta hanyar haɗakar Binciken-Anchor Synthesis (cPAS).

Sabis ɗin jerin laburaren da aka yi da aka riga aka yi yana sauƙaƙe abokan ciniki wajen shirya dakunan karatu daga mabambanta (mRNA, gabaɗayan kwayoyin halitta, amplicon, da sauransu).Daga baya, waɗannan ɗakunan karatu za a iya jigilar su zuwa cibiyoyin jerin abubuwan mu don kula da inganci da tsari a dandamalin Illumina ko BGI.