● Cikakken fakitin nazari, yana ƙunshe da bincike takwas da ake buƙata
● Babban dogaro a cikin bincike tare da cikakken bayani da sauƙin fahimta akan sakamako
● Ƙirar da aka tsara da kyau don bugawa
● Ƙwararrun ƙwararrun ƙungiyar bioinformatics suna cika buƙatun bincike na keɓaɓɓu daban-daban
● Gajeren lokacin juyawa tare da daidaito mafi girma a cikin bincike
● Ƙwarewa mai yawa tare da shari'o'in nasara sama da 90 tare da tarin tasirin tasirin da aka buga sama da 900
Kiyasin lokacin juyawa | Yawan nau'in | Yin nazari |
30 kwanakin aiki | 6-12 | Tarin iyali Fadada iyali da ƙanƙancewa Gina bishiyar phylogenetic Ƙimar lokacin rarrabuwar kawuna (ana buƙatar daidaitawar burbushin halittu) Lokacin shigar LTR (Na tsire-tsire) Kwafin kwayoyin halitta gaba daya (Na tsirrai) Matsin zaɓi Synteny bincike |
● Iyalin Halittu
● Physogenetics
● Lokacin rarrabuwa
● Zaɓin matsa lamba
● Nazarin synteny
Don Tissue
Nau'o'i | Nama | Bincike | Farashin CCS |
Dabba | Visceral nama | 0.5 ~ 1 g | 3.5g |
Naman tsoka | |||
5.0 g | |||
≥ 5.0ml | |||
Jinin dabbobi masu shayarwa | |||
0.5 ml na ruwa | |||
Jinin kaji/Kifi | |||
Shuka | Fresh Leaf | 1 ~2g ku | 5.0 g |
Petal/Stem | 1 ~2g ku | ≥ 10.0 g | |
Tushen/Iri | 1 ~2g ku | ≥ 20.0 g | |
Kwayoyin halitta | Tantanin halitta | - | 1 x 108 |
Fayilolin jerin kwayoyin halitta (.fasta) da fayilolin annotation (.gff3) na nau'ikan nau'ikan da ke da alaƙa
*Sakamakon demo da aka nuna anan duk daga kwayoyin halittar da aka buga tare da Biomarker Technologies
1.LTR saka kimar lokaci: Hoton ya nuna keɓantaccen rarraba bimodal a cikin lokutan saka LTR-RTs a cikin nau'in hatsin rai na Weining, idan aka kwatanta da sauran nau'ikan.Kololuwar kwanan nan ta bayyana kusan shekaru miliyan 0.5 da suka wuce.
Li Guang et al.,Halitta Halitta, 2021
2. An samo irin nazarin kwayar halitta akan chayote (Sechium Edule): ta hanyar nazarin nau'ikan mutane 13 a cikin dangin Ginire, an gano cewa Annaguua).Chayote da aka samo daga gourd maciji a cikin kusan 27-45 Mya da dukan kwayoyin halitta (WGD) an lura dasu a cikin chayote a cikin 25± 4 Mya, wanda shine taron WGD na uku a cikin cucuibitaceae.
Fu A da al.,Binciken Horticulture, 2021
3.Synteny analysis: Wasu kwayoyin halittar da ke da alaƙa da phytohormones a cikin haɓakar 'ya'yan itace an samo su a cikin chayote, gourd maciji da squash.Dangantaka tsakanin chayote da squash ya ɗanɗana sama da na tsakanin chayote da gourn maciji.
Fu A da al.,Binciken Horticulture, 2021
4.Gene bincike na iyali: KEGG haɓakawa akan haɓakar dangin iyali da raguwa a cikin G.thurberi da G.davidsonii genomes sun nuna cewa kwayoyin halitta na steroid biosynthesis da brassinosteroid biosynthesis sun haɓaka.
Yang Z et al.,BMC Biology, 2021
5.Dukkan nazarin kwafi na genome: 4DTV da nazarin rarraba Ks sun nuna duk taron kwafi na genome.Kololuwa na intraspecies da aka nuna abubuwan da suka dace.Kololuwar interspecies an nuna abubuwan da suka faru ta musamman.Nazarin ya nuna cewa yana kwatanta tare da sauran jinsunan da ke da alaƙa da uku, O. Europaea ya bi babban sikelin grale da kwanan nan.
Rao G et al.,Binciken Horticulture, 2021
BMK Case
Rose ba tare da prickle ba: fahimtar kwayoyin halitta da ke da alaƙa da daidaitawar danshi
Buga: Binciken Kimiyya na Ƙasa, 2021
Dabarun tsarawa:
'Basye'sMara ƙaya(R.Wichurainangenome:
Kimanin93 X PacBio + kusan.90 X Nanopore + 267 X Illumina
Sakamako mai mahimmanci
1.An gina babban ingancin R.wichuraiana genome ta hanyar amfani da dabarun da aka dade ana karantawa, wanda ke samar da taro na 530.07 Mb (Kimanin girman genome ya kusan 525.9 Mb ta hanyar cytometry kwarara da 525.5 ta hanyar binciken genome; Heterozygosity yana kusa da 1.03%).BUSCO ta kiyasta maki 93.9%.Idan aka kwatanta da "Tsohuwar blush" (haploOB), inganci da cikar wannan kwayar halitta an tabbatar da su ta hanyar daidaitattun tushe guda ɗaya da ma'aunin taro na LTR (LAI=20.03).R.wichuraiana genome ya ƙunshi ƙwayoyin furotin 32,674.
2.Multi-omics haɗin gwiwa bincike, wanda ya ƙunshi kwatancen genomics, transcriptomics, QTL bincike na yawan kwayoyin halitta, ya bayyana mahimmin ƙira tsakanin R. wichuraiana da Rosa chinensis.Hakanan, bambancin magana na kwayoyin halitta masu alaƙa a cikin QTL ana iya danganta su da ƙirar tushe.
Kwatankwacin ilimin genomics anaysis tsakanin Basye;s Thornless da Rosa chinensis ciki har da nazarin synteny, gungu na iyali, fadadawa da bincike na ƙanƙancewa, ya bayyana ɗimbin bambance-bambance, waɗanda ke da alaƙa da mahimman halaye a cikin wardi.Faɗawa na musamman a cikin dangin NAC da FAR1/FRS ana iya danganta su da juriya ga tabo baƙar fata.
Binciken kwatankwacin genomics tsakanin BT da haploOB genomes.
Zhong, M., da dai sauransu."Rose ba tare da prickle ba: fahimtar genomic da ke da alaƙa da daidaitawar danshi"Binciken Kimiyya na Ƙasa, 2021;, nnwab092.