BMKMANU S1000 Spatial Transcriptome
Tsarin fasaha na BMKMANU S1000 Spatial Transcriptome
Amfanin BMKMANU S1000
1) Ƙaddamar da ƙananan salula: Kowane yanki na kama yana ƙunshe da> 2 miliyan Barcoded Spots na sarari tare da diamita na 2.5 µm da tazara na 5 μm tsakanin wuraren tabo, yana ba da damar nazarin bayanan sararin samaniya tare da ƙudurin sub-cellular (5 μm).
2) Ƙididdigar ƙuduri mai yawa: bincike mai sauƙi mai sauƙi wanda ya fito daga 100 μm zuwa 5 μm don warware nau'o'in nau'in nama a mafi kyawun ƙuduri.
3) Cikakkun bayanan bayanan da aka rubuta: Za a iya tantance kwafin kwafin da aka ɗauka daga gabaɗayan faifan nama, ba tare da ƙuntatawa kan adadin ƙwayoyin halittar da aka yi niyya ba.
Ƙayyadaddun Sabis
| Laburare | Dabarun jeri | An ba da shawarar fitar da bayanai |
| S1000 cDNA ɗakin karatu | BMKMANU S1000-Illumina PE150 | 60Gb/samfuri |
Samfuran Bukatun
| Misali | Lamba | Girman | RNA ingancin |
| OCT toshe nama | 2-3 tubalan / samfurin | Kimanin6.8x6.8x6.8mm3 | RIN≥7 |
Don ƙarin cikakkun bayanai kan jagorar shirye-shiryen samfuri da aikin sabis, da fatan za a ji daɗin magana da aMasanin BMKGENE
Gudun Aikin Sabis
Gwajin ƙira
Samfurin bayarwa
Pre-gwaji
Barcoding na sarari
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
Ana nazarin bayanan da BMKMANU S1000 ya ƙirƙira ta hanyar amfani da software "BSTMatrix", wanda BMKGENE ya tsara ta kansa, gami da:
1) Ƙwararrun matrix na magana mai ƙarfi
2) HE image sarrafa
3) Mai jituwa tare da software na ɓangare na uku na ƙasa don bincike
4) Kan layi "BSTViewer" yana taimakawa don samun sakamakon gani a matakai daban-daban.
1.Tarar tabo
2.Spatial rarraba
Note: Shawaramatakin = 13 (100 µm, hagu); 7 (50 µm, dama)
3.Map mai nuna alama yawan taswirar zafi
4.Inter-sample data analysis
