Bacteria Complete Genome

Kwayoyin Cikakkun Halittun Halitta da Aka Fitar da Hoton

Biomarker Technologies yana ba da sabis na jeri akan gina cikakkiyar kwayar halittar ƙwayoyin cuta tare da gibin sifili.Babban aikin ƙwayoyin cuta cikakken ginin kwayoyin halitta ya haɗa da jerin tsararrun tsararraki na uku, taro, bayanin aiki da ci-gaba na nazarin halittu masu cika takamaiman manufofin bincike.Ƙarin cikakkun bayanan ƙwayoyin cuta na kwayoyin halitta yana ba da damar bayyana mahimman hanyoyin da ke ƙarƙashin tsarin rayuwarsu, wanda kuma zai iya ba da mahimmanci ga bincike na kwayoyin halitta a cikin nau'in eukaryotic mafi girma.

Dandalin:Nanopore PromethION P48 + Illumina NovaSeq Platform

PacBio Sequel II

Tuntube Mu

Cikakkun Sabis

Sakamakon Demo

Amfanin Sabis

● Dabarun jeri da yawa akwai don maƙasudin bincike daban-daban

● Kwayoyin cikakken kwayoyin halitta tare da garantin 0 Gap.

● Ƙwarewa sosai a cikin ƙungiyar kwayoyin halittar kwayoyin cuta tare da fiye da 10,000 cikakkun kwayoyin halitta.

● Ƙwararrun goyon bayan fasaha na goyon bayan sana'a wanda ke cika ƙarin takamaiman bukatun bincike.

Ƙayyadaddun Sabis

JeriDabarun

Laburare

Garanti mai inganci

Kiyasin lokacin juyawa

Nanopore 100X + Illumina 50X

Nanopore 20K

PE150

0 Gap

Kwanaki 30

PacBio HiFi 30X

PacBio 10K

Binciken bioinformatics

● Raw data ingancin kula

● Taron al'ada

● Binciken bangaren kwayoyin halitta

● Bayanin aikin Halittu

● Kwatankwacin nazarin kwayoyin halitta

Samfuran Bukatun da Bayarwa

Samfuran Bukatun:

DominCire DNA:

Nau'in Misali	Adadin	Hankali	Tsafta
Cire DNA	ku 2 μg	20 ng/ml	OD260/280= 1.6-2.5

Don samfuran nama:

Nau'in samfurin

Nasihar samfurin magani

Samfurin ajiya da jigilar kaya

Kwayoyin cuta

Kula da kwayoyin cuta a karkashin na'urar gani da ido kuma tattara kwayoyin cuta a cikin lokacin da suka wuce

Canja wurin al'adun ƙwayoyin cuta (wanda ke ɗauke da kusan sel 3-4.5e9) zuwa 1.5 ko 2 ml eppendorf.(Ci gaba akan kankara)

Centrifuge bututu na 1 min a 14000 g don tattara ƙwayoyin cuta kuma a cire abin da ke sama a hankali.

Rufe bututun kuma daskare ƙwayoyin cuta a cikin ruwa nitrogen na akalla minti 30.Ajiye bututu a cikin firiji -80 ℃.

Daskare samfuran a cikin ruwa nitrogen na sa'o'i 3-4 kuma adana a cikin ruwa nitrogen ko -80 digiri zuwa ajiyar dogon lokaci.Ana buƙatar jigilar samfuri tare da bushe-kankara.