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Ƙididdiga don Canjawa-Sarfafa Chromatin tare da Babban Sabis na Sabis (ATAC-seq)

ATAC-seq hanya ce mai mahimmanci don yin nazari akan samun damar chromatin mai fa'ida ga kwayoyin halitta, wanda ke da mahimmanci ga kula da epigenetic na duniya na maganganun kwayoyin halitta.Ana shigar da adaftan jeri cikin buɗaɗɗen yankuna na chromatin ta hanyar transposase mai ƙarfi Tn5.Bayan haɓakawa na PCR, ana gina ɗakin karatu na jerin abubuwa.Ana iya samun duk yankuna na chromatin da ke buɗe ƙarƙashin takamaiman yanayin lokacin sarari, ba kawai iyakance ga wuraren ɗaure na abin rubutu ba, ko wani yanki da aka gyara na tarihi.


Cikakkun Sabis

Bioinformatics

Sakamakon Demo

Amfanin Sabis

● Cikakken Bincike: Bututun bincike na ƙwararru don saduwa da buƙatun bincike na musamman;

● Ƙwarewar Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙadda tọn tọn na Ƙaddamarwa na Ƙarfafawa na Ƙarfafa Ɗaukaka ) da aka kammala ya zuwa yanzu, ciki har da samfurori na dabba da tsire-tsire.

Ƙayyadaddun Sabis

Platform: Illumina NovaSeq dandamali

Nau'in ɗakin karatu: ATAC-seq

Fitowar bayanai da aka ba da shawarar: ≥20M ya karanta

Samfuran Bukatun

Nau'in Samfura: Nama, sel masu rai, ko daskararre sel da sauransu

Lambar salula: ≥ 5×105sel, cikakken lambar tantanin halitta shine mabuɗin nasarar gwajin;

Nauyin nama: ≥ 200mg sabo ne nama;

Jini: ≥ 2 ml

Gudun Aikin Sabis

Misalin QC

Gwajin ƙira

samfurin bayarwa

Samfurin bayarwa

Gwajin matukin jirgi

Cire DNA

Shirye-shiryen Laburare

Gina ɗakin karatu

Jeri

Jeri

Binciken bayanai

Binciken bayanai

Bayan Sabis na siyarwa

Bayan-sayar da sabis


  • Na baya:
  • Na gaba:

  • 表观-02

    1.Heatmap akan ATAC yana karanta rarrabawa a TSS da yankuna kusa (± 3 kb)

    3 (1)2.Rarraba yankin bude chromatin a cikin sassa daban-daban na genome

    4 (1)

    3.Bambancin kololuwar kira tsakanin kungiyoyi

    5 (1)

    samun zance

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