10x Genomics Visium Spatial Transcriptome
Siffofin
● Ƙaddamarwa: 100 µM
● Tabo Diamita: 55 µM
● Yawan tabo: 4992
● Yankin ɗauka: 6.5 x 6.5 mm
● Kowane tabo da aka lissafta ana ɗora shi da maƙaloli da suka ƙunshi sassa 4:
- wutsiya poly(dT) don ƙaddamar da mRNA da haɗin cDNA
- Mai Gano Kwayoyin Halitta na Musamman (UMI) don gyara son zuciya na haɓakawa
- Barcode na sarari
- Daure jerin juzu'i na karanta juzu'i 1 na farko
● Tabon sassan H&E
Amfani
●Sabis na tsayawa ɗaya: ya haɗu da duk ƙwarewa da matakai na tushen fasaha, ciki har da sashin cryo-sectioning, tabo, haɓaka nama, ƙirar sararin samaniya, shirye-shiryen ɗakin karatu, sequencing da bioinformatics.
● Ƙwararrun ƙwararrun ƙungiyar fasaha: tare da gogewa a cikin nau'ikan nama sama da 250 da nau'ikan 100+ da suka haɗa da ɗan adam, linzamin kwamfuta, dabbobi masu shayarwa, kifi da tsirrai.
●Sabuntawa na ainihi akan duk aikin: tare da cikakken iko na ci gaban gwaji.
●Cikakken daidaitattun bioinformatics:kunshin ya ƙunshi nazarin 29 da ƙididdiga masu inganci 100+.
●Kirkirar bayanai da hangen nesa: akwai don buƙatun bincike daban-daban.
●Binciken haɗin gwiwa na zaɓi tare da jeri na mRNA guda-cell
Ƙayyadaddun bayanai
| Samfuran Bukatun | Laburare | Dabarun jeri | An ba da shawarar bayanai | Kula da inganci |
| Samfuran cryo da aka haɗa da OCT, samfuran FFPE (Mafi kyawun diamita: kimanin 6x6x6 mm3) 3 tubalan kowane samfurin | 10X Visium cDNA ɗakin karatu | Farashin PE150 | 50K PE yana karantawa kowane tabo (60Gb) | RIN> 7 |
Don ƙarin cikakkun bayanai kan jagorar shirye-shiryen samfuri da aikin sabis, da fatan za a ji daɗin magana da aMasanin BMKGENE
Gudun Aikin Sabis
A cikin lokacin shirye-shiryen samfurin, ana yin gwajin haƙar RNA na farko don tabbatar da samun RNA mai inganci.A cikin matakin inganta kyallen jikin sassan sassan suna tabo kuma an duba su kuma an inganta yanayin ƙetare don sakin mRNA daga nama.Sannan ana amfani da ingantacciyar ƙa'idar yayin aikin ginin ɗakin karatu, sannan a bi da bi da kuma nazarin bayanai.
Cikakken aikin sabis ɗin ya haɗa da sabuntawa na ainihi da kuma tabbatarwa abokin ciniki don kula da madaidaicin amsawa, yana tabbatar da aiwatar da aikin mai santsi.
Ya haɗa da bincike mai zuwa:
Kula da ingancin bayanai:
o Fitowar bayanai da rarraba makin inganci
o Gano kwayoyin halitta a kowane tabo
o Ciwon nama
Binciken samfurin ciki:
o wadatar halittu
o Tarin tabo, gami da raguwar ƙididdigar girma
o Binciken bambancin magana tsakanin tari: gano kwayoyin halitta masu alama
o Bayanin aiki da haɓaka kwayoyin halitta masu alama
Binciken Inter-group
o Sake haɗa tabo daga samfuran biyu (misali marasa lafiya da sarrafawa) da sake tari
o Gano kwayoyin halitta masu alama ga kowane tari
o Bayanin aiki da haɓaka kwayoyin halitta masu alama
o Bambance-bambancen gungu iri ɗaya tsakanin ƙungiyoyi
Nazari-samfurin ciki
Tari tari
Gano kwayoyin halitta masu alamar alama da rarraba sararin samaniya
Binciken Inter-group
Haɗin bayanai daga ƙungiyoyin biyu da sake tari
Alamar kwayoyin halittar sabbin tari
Bincika ci gaban da sabis na fassarorin sararin samaniya na BMKGene ya samu ta hanyar 10X Visium A cikin waɗannan fitattun littattafan:
Chen, D. et al.(2023) 'mthl1, yuwuwar Drosophila homologue na mammalian adhesion GPCRs, yana da hannu a cikin halayen antitumor zuwa alluran ƙwayoyin oncogenic a cikin kwari', Gabatarwa na Cibiyar Nazarin Kimiyya ta Ƙasar Amurka ta Amurka, 120(30), p.e2303462120.doi: /10.1073/pnas.2303462120
Chen, Y. et al.(2023)' KARFE yana ba da damar ƙididdige ƙididdiga masu ƙima na bayanan kwafi na spatiotemporal', Briefings in Bioinformatics, 24(2), shafi 1-10.doi: 10.1093/BIB/BBAD068.
Liu, C. et al.(2022) 'A spatiotemporal atlas na organogenesis a cikin ci gaban orchid furanni', Nucleic Acids Research, 50 (17), shafi. 9724-9737.doi: 10.1093/NAR/GKAC773.
Wang, J. et al.(2023) 'Haɗin Fassarar Fassarar Fassarar Fayil da Ƙaƙwalwar Ƙwararren Ƙwararren Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararru, 19 (8), shafi na 2515-2530.doi: 10.7150/IJBS.83510.
