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T2T GENOME ASSEMBLY, GAP FREE GENOME

1stTwa Rice Genomes1

Titel: Gearstalling en falidaasje fan twa gap-frije referinsjegenoom foar Xian / indica Rice ûntbleatet ynsjoch yn plant Centromere Architecture

Doi:https://doi.org/10.1101/2020.12.24.424073

Pleatst tiid: 01 jannewaris 2021.

Ynstitút: Huazhong Agricultural University, Sina

Materialen

O. sativa xian/indicarysrassen 'Zhenshan 97 (ZS97)' en 'Minghui 63 (MH63)

Sequencing strategy

NGS lêst + HiFi-lêzen + CLR-lêzen + BioNano + Hi-C

Data:

ZS97: 8.34 Gb(~23x) HiFi-lêzen + 48.39 Gb (~131x) CLR-lêzen + 25 Gb(~69x) NGS + 2 BioNano Irys-sellen

MH63: 37,88 Gb (~103x) HiFi-lêzen + 48,97 Gb (~132x) CLR-lêzen + 28 Gb (~76x) NGS + 2 BioNano Irys-sellen

Figuer 1

Ofbylding 1 Twa gap-frije genomen fan rys (MH63 en ZS97)

2ndBanana Genome2

Titel: Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing

Doi:https://doi.org/10.1101/2021.04.16.440017

Tiid pleatst: 17 april 2021.

Ynstitút: Université Paris-Saclay, Frankryk

Materialen

Dûbel haploidMusa acuminatasppmalacensis(DH-Pahang)

Sequence strategy en gegevens:

HiSeq2500 PE250 modus + MinION/ PromethION (93Gb,~200X)+ Optyske kaart (DLE-1+BspQ1)

Tabel 1 Ferliking fan Musa acuminata (DH-Pahang) genome gearkomsten

Tabel 1-Fergeliking-fan-GRCh38-en-T2T-CHM13-minsklike-genoom-gearkomsten
Figure-Musa-genomes-arsjitektuer-fergeliking

figuer 2 Musa genomes arsjitektuer ferliking

3rdPhaeodactylum tricornutum genome3

Titel: Telomere-to-telomere genome gearstalling fanP
haeodactylum tricornutum

Doi:https://doi.org/10.1101/2021.05.04.442596

Pleatst tiid: 04 maaie 2021

Ynstitút: Western University, Kanada

Materialen

Phaeodactylum tricornutum(Culture Collection of Algae and Protozoa CCAP 1055/1)

Sequence strategy en gegevens:

1 Oxford Nanopore minION flow sel + in 2 × 75 paired-end mid-output NextSeq 550 run

Figure-Workflow-for-telomere-to-telomere-genome-assembly-1-1024x740

Ofbylding 3 Workflow foar telomere-to-telomere genome-assemblage

4thHuman CHM13 genoom4

Titel: De folsleine folchoarder fan in minsklik genoom

Doi:https://doi.org/10.1101/2021.05.26.445798

Pleatst tiid: 27 maaie 2021

Ynstitút: National Institutes of Health (NIH), Feriene Steaten

Materiaal: selline CHM13

Sequence strategy en gegevens:

30 × PacBio sirkulêre konsensus-sequencing (HiFi), 120 × Oxford Nanopore ultra-lange lêssekwinsje, 100 × Illumina PCR-frije sequencing (ILMN), 70 × Illumina / Arima Genomics Hi-C (Hi-C), BioNano Genomics Hi-C (Hi-C) en Strand-seq

Tabel 2 Fergeliking fan GRCh38 en T2T-CHM13 minsklike genome assemblies

Tabel-fergeliking-fan-Musa-acuminata-DH-Pahang-genoom-gearkomsten

Referinsje

1. Sergey Nurk et al.De folsleine folchoarder fan in minsklik genoom.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798

2. Caroline Belser et al.Telomere-to-telomere gapless chromosomen fan banaan mei help fan nanopore sequencing.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017

3. Daniel J. Giguere et al.Telomere-to-telomere genome assembly of Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596

4. Jia-Ming Song et al.Gearstalling en falidaasje fan twa Gap-frije referinsje genomes foar Xian / indica Rice ûntbleatet ynsjoch yn Plant Centromere Architecture.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073


Post tiid: Jan-06-2022

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