The article, “MYC2 Orchestrates a Hierarchical Transcriptional Cascade That Regulates Jasmonate-Mediated Plant Immunity in Tomato”, was published in The Plant Cell.
This study shows that the basic helix-loop-helix transcription factor (TF) MYC2 in tomato (Solanum lycopersicum) acts downstream of the hormone jasmonate (JA) receptor to orchestrate JA-mediated activation of both the wounding and pathogen responses.
Using chromatin immunoprecipitation sequencing (ChIP-seq) coupled with RNA sequencing (RNA-seq) assays identified 655 MYC2-targeted JA-responsive genes. These findings suggest that MYC2 and its downstream MTFs form a hierarchical transcriptional cascade during JA-mediated plant immunity that initiates and amplifies transcriptional output.
As proof of concept, we showed that during plant resistance to the necrotrophic pathogen Botrytis cinerea, MYC2 and the MTF JA2-Like form a transcription module that preferentially regulates wounding-responsive genes, whereas MYC2 and the MTF ETHYLENE RESPONSE FACTOR.
BMKGENE provided sequencing and analysis services of RNA and ChIP-seq for this study.
If you would like to learn more about this study, access this link. For more information on our sequencing and bioinformatics services, you can talk to us here.
Post time: Jul-23-2024