BMKGENE provided ONT long-read nanopore RNA sequencing and ATAC-seq service for the study “Epigenetic and transcriptional activation of the secretory kinase FAM20C as an oncogene in glioma”, which was published in the 《Journal of Genetics and Genomics》.
This study constructed the full-length transcriptome atlas in paired gliomas and observed that 22 genes are upregulated by full-length transcriptome and differential APA analysis. Analysis of ATAC-seq data reveals that both FAM20C and NPTN are the hub genes with chromatin openness and differential expression.
Further, in vitro and in vivo studies suggest that FAM20C stimulates the proliferation and metastasis of glioma cells. Meanwhile, NPTN, a novel cancer suppressor gene, counteracts the function of FAM20C by inhibiting both the proliferation and migration of glioma. The blockade of FAM20C by neutralizing antibodies results in the regression of xenograft tumors. Moreover, MAX, BRD4, MYC, and REST are found to be the potential trans-active factors for the regulation of FAM20C.
Taken together, these results uncover the oncogenic role of FAM20C in glioma and shed new light on the treatment of glioma by abolishing FAM20C .
Click here to learn more about this study
Post time: Oct-20-2023