The article published in Journal Of General Virology, Dicer-2 mutations in Aedes aegypti cells lead to a diminished antiviral function against Rift Valley fever virus and Bunyamwera virus infection. Mosquitoes are known to transmit different arthropod-borne viruses belonging to various virus families. The exogenous small interfering RNA pathway plays an important role in the mosquito defence against such virus infections, with Dicer-2 (Dcr2) as one of the key proteins that initiates the cleavage of viral dsRNAs into 21 nt long virus-derived small interfering RNAs. Previous data identified the importance of various motifs in Dcr2 for its small interfering RNA (siRNA)-mediated antiviral activity.
However, all these data focus on positive-strand RNA viruses, although negative-strand RNA viruses, like Bunyaviricetes, include several important mosquito-borne viruses. Here, this study aims to investigate the importance of different domains of Dcr2 for antiviral activity against viruses of the Bunyaviricetes. All used domains were determined to be critical for the antiviral activity against Bunyaviricetes.
BMKGENE provided small RNA sequencing and bioinformatics analysis services for this study.
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Post time: Dec-10-2024