BMKGENE provided full-length transcriptome sequencing services using PacBio and ONT technologies for a study titled “Comparative analysis of PacBio and ONT RNA sequencing methods for Nemopilema Nomurai venom identification”, which was published in the journal Genomics. The study aimed to compare the effectiveness of PacBio and ONT RNA sequencing methods in identifying the venom of the jellyfish species Nemopilema nomurai.
The findings of the study suggest that ONT generally produced higher raw data quality in the transcriptome analysis, while PacBio generated longer read lengths. PacBio was found to be superior in identifying coding sequences and long-chain noncoding RNA, whereas ONT was more cost-effective for predicting alternative splicing events, simple sequence repeats, and transcription factors.
This study has significant implications for future sequencing technologies in marine jellyfish and highlights the power of full-length transcriptome analysis in discovering potential therapeutic targets for jellyfish dermatitis.
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Post time: Dec-20-2023