Delve into the potential of studying wild variety of research species, exemplified by a pivotal case from BMKGENE’s clients. Recently published in this year’s edition of the Plant Biotechnology Journal , the article titled “Chromosome-level Wild Hevea brasiliensis Genome: Empowering Genomic-Assisted Breeding and Unearthing Vital Loci for Elevated Rubber Yield” stands as a valuable reference.
The Brazilian rubber tree (Hevea brasiliensis) stands tall as a pivotal natural rubber source. Throughout the last century, traditional breeding methodologies triggered a remarkable sixfold surge in rubber production. Yet, the underlying genetic bedrock responsible for potential rubber yield enhancement remains enigmatic.
This study embarked on constructing a chromosome-level genome for the wild rubber tree, employing a synergy of Nanopore long-read sequencing, Illumina NGS sequencing, and Hi-C technology.
In parallel, a trove of 147 germplasm resources, encompassing high-yield variations, wild strains, and related species, was assembled for comprehensive whole genome resequencing (WGS) using the Illumina platform. The ensuing sequencing data underwent rigorous population genetics analysis, enabling the dissection of population structure, diversity, and linkage disequilibrium. This analysis shed light on domestication variations across populations.
Further investigations encompassed analysis encompassing Fst, π, Tajima’s D, and LD, aiming to unveil selection signals arising from improved latex yield, notably focusing on Wickham clones.
Culminating in a Genome-wide Association Analysis (GWAS), markers linked to yield traits and subsequently yield-related genes were pinpointed. Initial validation unveiled distinctive transcriptional activities across different mutants for two ERF genes, providing a preliminary glimpse into their functional significance. Further corroboration is scheduled within forthcoming research.
BMKGENE takes pride in contributing to the extensive whole genome resequencing of this sizable population, along with a substantial role in the bioinformatics analysis. Our vast experience encompasses NGS/TGS sequencing and multiomics bioinformatics analysis across an array of 1000+ species. We look forward to the prospect of partnering with you on your upcoming projects.
Click here to learn more about this study
Post time: Aug-29-2023