BMKGENE provided spatial transcriptomics sequencing services for this study: a potential Drosophila homologue of mammalian adhesion GPCRs, is involved in antitumor reactions to injected oncogenic cells in flies, which was published in PNAS, mthl1.
In this study, adult male flies were injected with OCs, and spatial transcriptome sequencing on frozen flies are performed. The injection of OCs into adult male flies induces a strong transcriptomic response in the host flies featuring in particular genes encoding bona fide G-coupled proteins, among which the gene for methuselah-like 1 is prominent.
Through a combination of genetic manipulations of the mthl1 gene (loss of function and overexpression of mthl1), documented that indeed this gene has an antiproliferative effect. Further, show that mthl1 controls the expression of a large number of genes coding for chemoreceptors and genes implicated in the regulation of development. Deciphered the defense mechanisms of flies against tumors in the absence of adaptive immunity.
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Post time: Aug-10-2023