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Factory wholesale Dna Sequencing Analysis - Whole genome bisulfite sequencing – Biomarker
Service Advantages Sample Requirements Service Specifications Platform: NovaSeq 6000 platform, PE150 Recommended Sequencing Depth :≥30× coverage Service Work Flow Experiment design Sample delivery DNA extraction Library construction Sequencing Data analysis After-sale services
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Factory Cheap Hot Srna Sequencing - Metatranscriptome Sequencing – Biomarker
Sample Requirements Sample types: Total RNA; Amount: 2.5ug; Concentration:50ng/ul; Services Strategy Library Type: 250-300bp insert cDNA library; Sequencing Platform and Strategy: illumine NovaSeq 6000;paired-end 150bp; Recommended Data Output: 12G raw data/sample Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale services
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Professional Design 10x Genomics Cost - small RNA – Biomarker
Bioinformatics Raw data quality control Common and unique sequence among samples Small RNA classification Alignment with reference genome (+/- strands) Genome-wide smallRNA distribution genes Known miRNA identification Small RNA annotation (miRBASE, RepBASE, Rfam, SILVA, GtRNAdb, etc.) miRNA base perference miRNA base editing ...
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Wholesale Sequencing Strategy - GWAS – Biomarker
Bioinformatics Raw data quality control Alignment against reference genome Genome-wide variation identification Evolutional genetics analysis Genotype imputation (lower than 3X) SNP-traits association study Indel-traits association study Candidate gene annotation LD-block analysis on significant GWAS Associa...
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Super Lowest Price Transcriptome Sequencing Service - Eukaryotic mRNA sequencing – Illumina – Biomarker
Advantages Sample Requirements and Delivery Library Sequencing strategy Data recommended Quality Control Poly A enriched Illumina PE150 6 Gb Q30≥85% Sample Requirements: Nucleotides: Purity Integrity Amount OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg Tissue: Weight(dry): ≥1 g *For t...
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Factory source Transcriptome - Bacteria Complete Genome – Biomarker
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Chinese wholesale 18s Sequencing Cost - Metagenomics (NGS) – Biomarker
Transcipt identification Raw data processing Metagenome assembly Metagenome component analysis PCA Function annotation Alpha diversity analysis -Gene level Beta diversity on functional genes PCoA Non-redundant gene set NMDS UPGMA Anosim permanova RDA/CCA Intergroup differential analysis Alpha diversity analysis -Species level: Random forest Lefse Beta diversity analysis on species
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High definition 16s Rrna Gene Sequencing - mRNA(Reference) – Biomarker
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18 Years Factory Transcriptome Analysis Methods - circRNA sequencing – Illumina – Biomarker
Service Advantages Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Integrity Contamination Amount OD260/280≥1.7-2.5;OD260/230≥0.5-2.5; For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation Limited or no protein or DNA contamination shown on gel. Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg Tissue: Weight(dry): ≥1 g *For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue ...
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China Gold Supplier for Variation Detection - DNA sequencing -PacBio Sequencer – Biomarker
Features Two sequencing mode on PacBio sequencer: CLR and CCS. Mode Library Data yield Single-base accuracy Applications CLR 20Kb, 30Kb, etc. 80 Gb to 130 Gb/cell Approx. 85% De novo, SV calling, etc. CCS 15-20 Kb 14-40 Gb/cell (CCS) Approx. 99% De novo, Iso-Seq, SNP/Indel/SV calling Service Advantages Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Appearance Electrophoresis Gel Amount Others OD260/280=1.6-2.3; OD260/230=...
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High reputation Rna Seq - Long non-coding sequencing-Illumina – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Whole genome resequencing ü Data processing ü SNP/Indel calling ü Candidate region screening ü Candidate gene function annotation Sample Requirements and Delivery Sample Requirements: gDNA sample Tissue sample Concentration: ≥30 ng/μl Plants: 1-2 g Amount: ≥2 μg (Volumn ≥15 μl) Animals: 0.5-1 g Purity: OD260/280= 1.6-2.5 Whole blood: 1.5 ml Service Work Flow ...
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Top Suppliers Mirna Sequencing Service - Metabolomics – Biomarker
Sample Requirements Non-targeted metabolomics Sample types Recommended sample amount Biological Repetition LC-MS/GC-MS untargeted metabolomics Tissues 200mg Plant ≥6; Animal ≥10; Human ≥30; Plasma/serum 200ul Fecal/Intestinal Contents 150mg Cells 1*106 urine 500ul Rumen fluid 1ml Targeted metabolomics Sample types Recommended sample amount Biological Repetition Phytohormone/Tryptophane/Energy Metabolism/ Oxidized lipids/ Caroteno...
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China wholesale Bioinformatics Provider - circ-RNA – Biomarker
Bioinformatics Raw data quality control Reference genome alignment CircRNA identification circRNA locus circRNA conservation circRNA expression Novel circRNA prediction circRNA alternative splicing circRNA differential expression analysis circRNA-mRNA expression correlation Reproducibility test ...
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