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Quality Inspection for Metabarcoding Pricing - Amplicon (16S/18S/ITS) – Biomarker
Data Processing Raw data quality control Operational Taxonomic Unit(OTU) Clustering Taxonomy Species annotation Phylogenetic tree Components structure Alternative splicing Heatmap on species abundance Alpha Diversity Alpha-diversity metrics Rarefaction curve Shannon-Wiener curve Venn diagram Species accumulation curve Beta Diversity PCoA PCA UPGMA NMDS Advanced analysis Differential analysis LEfSe Intergroup correlation analysis Ternary phase diagram A... -
Good User Reputation for Chromosome-Scale Genome - Prokaryotic RNA sequencing – Biomarker
Service Specifications Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale services -
Factory making Strand-Specific Rna-Seq - Full-length mRNA sequencing -Nanopore – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Raw data processing ü Transcript identification ü Alternative splicing ü Expression quantification in gene level and isoform level ü Differential expression analysis ü Function annotation and enrichment (DEGs and DETs) Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Integrity Amount OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein o... -
2021 High quality Hifi Sequencing Provider - circ-RNA – Biomarker
Bioinformatics Raw data quality control Reference genome alignment CircRNA identification circRNA locus circRNA conservation circRNA expression Novel circRNA prediction circRNA alternative splicing circRNA differential expression analysis circRNA-mRNA expression correlation Reproducibility test ... -
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factory customized Bmk - Toolkits – Biomarker
Heatmap Drawer Heatmap drawer is used for heat map drawing, which can filter, normalize and cluster matrix data.It is mostly used for cluster analysis of gene expression level between different samples. Gene Annotation Attaching biological functions to sequences in FASTA file by aligning sequences to database, including NR, KEGG, COG, SwissProt, TrEMBL, KOG, Pfam. BLAST BLAST (Basic Local Alignment Search Tool) is an algorithm and program to find ... -
Professional China Alternative Splicing - Hi-C based Genome Assembly – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Raw data quality contro l ü Hi-C library quality control ü Hi-C based genome assembly ü Post-assembly evaluation Sample Requirements and Delivery Sample Requirements: For animals: 1.Muscle (Frozen): ≥ 2 g; 2.Whole blood (Non-frozen): ≥ 2 ml (for 3 libraries) For plants: 1.Seedlings (e.g. young seedlings cultivated from seeds, tissue culture seedingling, etc) : collect all leaves and young stem... -
PriceList for Shotgun Dna Sequencing - NGS-WGS (Illumina/BGI) – Biomarker
Bioinformatics Raw data quality control Alignment with reference genome SNP identification SNP annotation SNP calling among samples Small InDel identification Small Indels between sample SV calling Small InDel annotation CNV calling Distribution of variations on genome Circos map ... -
Personlized Products Microbial Genome - circRNA sequencing – Illumina – Biomarker
Service Advantages Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Integrity Contamination Amount OD260/280≥1.7-2.5;OD260/230≥0.5-2.5; For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation Limited or no protein or DNA contamination shown on gel. Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg Tissue: Weight(dry): ≥1 g *For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue ... -
2021 Good Quality 16s Rrna Sequencing - GWAS – Biomarker
Bioinformatics Raw data quality control Alignment against reference genome Genome-wide variation identification Evolutional genetics analysis Genotype imputation (lower than 3X) SNP-traits association study Indel-traits association study Candidate gene annotation LD-block analysis on significant GWAS Associa... -
Factory wholesale Shotgun Sequencing - Metabolomics – Biomarker
Sample Requirements Non-targeted metabolomics Sample types Recommended sample amount Biological Repetition LC-MS/GC-MS untargeted metabolomics Tissues 200mg Plant ≥6; Animal ≥10; Human ≥30; Plasma/serum 200ul Fecal/Intestinal Contents 150mg Cells 1*106 urine 500ul Rumen fluid 1ml Targeted metabolomics Sample types Recommended sample amount Biological Repetition Phytohormone/Tryptophane/Energy Metabolism/ Oxidized lipids/ Caroteno... -
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Original Factory Transcriptome Sequencing Provider - Bacterial and Fungal Whole Genome Re-sequencing – Biomarker
Service Specifications Platform: NovaSeq 6000 platform, PE150 Library type: 350bp insert cDNA library (depends on peak distribution) Sequencing strategy: Paired-End 150 bp Recommend sequencing coverage： ≥100× for bacterial genomes, ≥50× for fungal genomes Sample Requirements Genomic DNA Amount：≥ 3ug Genomic DNA Concentration：≥ 20ng/μL Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale ser...
