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Chinese wholesale 10x Genomics - Plant/Animal De novo Genome Sequencing – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Whole genome resequencing ü Data processing ü SNP/Indel calling ü Candidate region screening ü Candidate gene function annotation Sample Requirements and Delivery Sample Requirements: gDNA sample Tissue sample Concentration: ≥30 ng/μl Plants: 1-2 g Amount: ≥2 μg (Volumn ≥15 μl) Animals: 0.5-1 g Purity: OD260/280= 1.6-2.5 Whole blood: 1.5 ml Service Work Flow ...
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Quality Inspection for Function Annotation - Bacterial and Fungal Whole Genome Re-sequencing – Biomarker
Service Specifications Platform: NovaSeq 6000 platform, PE150 Library type: 350bp insert cDNA library (depends on peak distribution) Sequencing strategy: Paired-End 150 bp Recommend sequencing coverage: ≥100× for bacterial genomes, ≥50× for fungal genomes Sample Requirements Genomic DNA Amount:≥ 3ug Genomic DNA Concentration:≥ 20ng/μL Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale ser...
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OEM Factory for Ngs Service - DNA sequencing -PacBio Sequencer – Biomarker
Features Two sequencing mode on PacBio sequencer: CLR and CCS. Mode Library Data yield Single-base accuracy Applications CLR 20Kb, 30Kb, etc. 80 Gb to 130 Gb/cell Approx. 85% De novo, SV calling, etc. CCS 15-20 Kb 14-40 Gb/cell (CCS) Approx. 99% De novo, Iso-Seq, SNP/Indel/SV calling Service Advantages Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Appearance Electrophoresis Gel Amount Others OD260/280=1.6-2.3; OD260/230=...
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China New Product Shotgun Metagenomic Profiling - Prokaryotic RNA sequencing – Biomarker
Service Advantages Sample Requirements DNA quantity: ≥ 70 ng, main peak of 100 to 500 bp Sample volume: ≥ 10 μl OD260/280 = 1.8 to 2.0 without degradation or RNA contamination Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale services
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2021 Good Quality 16s Rrna Sequencing - Bulked Segregant analysis – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Whole genome resequencing ü Data processing ü SNP/Indel calling ü Candidate region screening ü Candidate gene function annotation Sample Requirements and Delivery Sample Requirements: gDNA sample Tissue sample Concentration: ≥30 ng/μl Plants: 1-2 g Amount: ≥2 μg (Volumn ≥15 μl) Animals: 0.5-1 g Purity: OD260/280= 1.6-2.5 Whole blood: 1.5 ml Service Work Flow ...
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Professional Design 10x Genomics Cost - Hi-C based Genome Assembly – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Raw data quality contro l ü Hi-C library quality control ü Hi-C based genome assembly ü Post-assembly evaluation Sample Requirements and Delivery Sample Requirements: For animals: 1.Muscle (Frozen): ≥ 2 g; 2.Whole blood (Non-frozen): ≥ 2 ml (for 3 libraries) For plants: 1.Seedlings (e.g. young seedlings cultivated from seeds, tissue culture seedingling, etc) : collect all leaves and young stem...
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China wholesale Wgs - LncRNA – Biomarker
Bioinformatics Raw data quality control Alignment with reference genome Transcript assembly Correlation analysis Known lncRNA identification Coding potential of candidate lncRNA Regulatory network: lncRNA-mRNA lncRNA expression quantification Differentially expressed lncRNA (DE-lncRNA) PCA WGCNA ...
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Cheapest Price Transcriptome Sequencing Cost - circRNA sequencing – Illumina – Biomarker
Service Advantages Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Integrity Contamination Amount OD260/280≥1.7-2.5;OD260/230≥0.5-2.5; For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation Limited or no protein or DNA contamination shown on gel. Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg Tissue: Weight(dry): ≥1 g *For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue ...
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Short Lead Time for Chip Seq Sequencing - Long non-coding sequencing – Illumina – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Whole genome resequencing ü Data processing ü SNP/Indel calling ü Candidate region screening ü Candidate gene function annotation Sample Requirements and Delivery Sample Requirements: gDNA sample Tissue sample Concentration: ≥30 ng/μl Plants: 1-2 g Amount: ≥2 μg (Volumn ≥15 μl) Animals: 0.5-1 g Purity: OD260/280= 1.6-2.5 Whole blood: 1.5 ml Service Work Flow ...
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OEM Factory for Ngs Service - Full-length mRNA sequencing -Nanopore – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Raw data processing ü Transcript identification ü Alternative splicing ü Expression quantification in gene level and isoform level ü Differential expression analysis ü Function annotation and enrichment (DEGs and DETs) Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Integrity Amount OD260/280≥1.8;OD260/230≥1.0;Clear peak at 260 nmLimited or no protein o...
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PriceList for Bioinformatics Companies - Nanopore-based metagenome sequencing – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Raw data quality control ü Metagenome assembly ü Non-redundant gene set and annotation ü Species diversity analysis ü Genetic function diversity analysis ü Inter-group analysis ü Association analysis against experimental factors Sample Requirements and Delivery Sample requirements and delivery Sample Requirements: For DNA extracts: Sample Type Amount Concentration Purity DNA ex...
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High Quality Mrna Analysis - Amplicon (16S/18S/ITS) – Biomarker
Data Processing Raw data quality control Operational Taxonomic Unit(OTU) Clustering Taxonomy Species annotation Phylogenetic tree Components structure Alternative splicing Heatmap on species abundance Alpha Diversity Alpha-diversity metrics Rarefaction curve Shannon-Wiener curve Venn diagram Species accumulation curve Beta Diversity PCoA PCA UPGMA NMDS Advanced analysis Differential analysis LEfSe Intergroup correlation analysis Ternary phase diagram A...
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Manufactur standard Next Gen Sequence - small RNA – Biomarker
Bioinformatics Raw data quality control Common and unique sequence among samples Small RNA classification Alignment with reference genome (+/- strands) Genome-wide smallRNA distribution genes Known miRNA identification Small RNA annotation (miRBASE, RepBASE, Rfam, SILVA, GtRNAdb, etc.) miRNA base perference miRNA base editing ...
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