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Service Specifications Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale services -
Leading Manufacturer for Eukaryotic Mrna - circ-RNA – Biomarker
Bioinformatics Raw data quality control Reference genome alignment CircRNA identification circRNA locus circRNA conservation circRNA expression Novel circRNA prediction circRNA alternative splicing circRNA differential expression analysis circRNA-mRNA expression correlation Reproducibility test ... -
Cheap price Rna Extraction - Prokaryotic RNA sequencing – Biomarker
Service Advantages Sample Requirements DNA quantity: ≥ 70 ng, main peak of 100 to 500 bp Sample volume: ≥ 10 μl OD260/280 = 1.8 to 2.0 without degradation or RNA contamination Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale services -
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Raw data procession CCS extraction Full-length non-chimeric(FLNC) reads identification Consensus transcripts identification Transcipt identification Unigenes construction Isoform identification Alternative splicing Differential alternative splicng APA analysis Differential APA Fusion genes Differential fu... -
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Service Advantages Sample Requirements Service Specifications Platform: NovaSeq 6000 platform, PE150 Recommended Sequencing Depth :≥30× coverage Service Work Flow Experiment design Sample delivery DNA extraction Library construction Sequencing Data analysis After-sale services -
Factory Cheap Hot Srna Sequencing - Metatranscriptome Sequencing – Biomarker
Sample Requirements Sample types: Total RNA； Amount: 2.5ug; Concentration:50ng/ul; Services Strategy Library Type： 250-300bp insert cDNA library； Sequencing Platform and Strategy: illumine NovaSeq 6000；paired-end 150bp; Recommended Data Output: 12G raw data/sample Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale services -
Professional Design 10x Genomics Cost - small RNA – Biomarker
Bioinformatics Raw data quality control Common and unique sequence among samples Small RNA classification Alignment with reference genome (+/- strands) Genome-wide smallRNA distribution genes Known miRNA identification Small RNA annotation (miRBASE, RepBASE, Rfam, SILVA, GtRNAdb, etc.) miRNA base perference miRNA base editing ... -
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Bioinformatics Raw data quality control Alignment against reference genome Genome-wide variation identification Evolutional genetics analysis Genotype imputation (lower than 3X) SNP-traits association study Indel-traits association study Candidate gene annotation LD-block analysis on significant GWAS Associa... -
Super Lowest Price Transcriptome Sequencing Service - Eukaryotic mRNA sequencing – Illumina – Biomarker
Advantages Sample Requirements and Delivery Library Sequencing strategy Data recommended Quality Control Poly A enriched Illumina PE150 6 Gb Q30≥85% Sample Requirements: Nucleotides: Purity Integrity Amount OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg Tissue: Weight(dry): ≥1 g *For t... -
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Transcipt identification Raw data processing Metagenome assembly Metagenome component analysis PCA Function annotation Alpha diversity analysis -Gene level Beta diversity on functional genes PCoA Non-redundant gene set NMDS UPGMA Anosim permanova RDA/CCA Intergroup differential analysis Alpha diversity analysis -Species level: Random forest Lefse Beta diversity analysis on species -
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18 Years Factory Transcriptome Analysis Methods - circRNA sequencing – Illumina – Biomarker
Service Advantages Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Integrity Contamination Amount OD260/280≥1.7-2.5;OD260/230≥0.5-2.5; For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation Limited or no protein or DNA contamination shown on gel. Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg Tissue: Weight(dry): ≥1 g *For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue ...
