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Good quality Genome Sequencing Company - Metagenomics (NGS) – Biomarker
Transcipt identification Raw data processing Metagenome assembly Metagenome component analysis PCA Function annotation Alpha diversity analysis -Gene level Beta diversity on functional genes PCoA Non-redundant gene set NMDS UPGMA Anosim permanova RDA/CCA Intergroup differential analysis Alpha diversity analysis -Species level: Random forest Lefse Beta diversity analysis on species -
Good Quality Whole Genome Sequencing - Chromatin Immunoprecipitation Sequencing (ChIP-seq) – Biomarker
Service Advantages Sample Requirements DNA quantity: ≥ 70 ng, main peak of 100 to 500 bp Sample volume: ≥ 10 μl OD260/280 = 1.8 to 2.0 without degradation or RNA contamination Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale services -
Factory Cheap Hot Genome Sequencing Companies - Eukaryotic mRNA sequencing – Illumina – Biomarker
Advantages Sample Requirements and Delivery Library Sequencing strategy Data recommended Quality Control Poly A enriched Illumina PE150 6 Gb Q30≥85% Sample Requirements: Nucleotides: Purity Integrity Amount OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg Tissue: Weight(dry): ≥1 g *For t... -
Good Quality Whole Genome Sequencing - Bacterial and Fungal Whole Genome Re-sequencing – Biomarker
Service Specifications Platform: NovaSeq 6000 platform, PE150 Library type: 350bp insert cDNA library (depends on peak distribution) Sequencing strategy: Paired-End 150 bp Recommend sequencing coverage： ≥100× for bacterial genomes, ≥50× for fungal genomes Sample Requirements Genomic DNA Amount：≥ 3ug Genomic DNA Concentration：≥ 20ng/μL Service Work Flow Sample delivery Library construction Sequencing Data analysis After-sale ser... -
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Factory wholesale Gwas Data Analysis - circRNA sequencing – Illumina – Biomarker
Service Advantages Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Integrity Contamination Amount OD260/280≥1.7-2.5;OD260/230≥0.5-2.5; For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation Limited or no protein or DNA contamination shown on gel. Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg Tissue: Weight(dry): ≥1 g *For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue ... -
2021 New Style Metagenome Sequencing - GWAS – Biomarker
Bioinformatics Raw data quality control Alignment against reference genome Genome-wide variation identification Evolutional genetics analysis Genotype imputation (lower than 3X) SNP-traits association study Indel-traits association study Candidate gene annotation LD-block analysis on significant GWAS Associa... -
China OEM Next Generation Sequencing Service - BSA – Biomarker
Bioinformatics Raw data quality control Reference genome alignment Tag clustering (non-ref) SNP and InDel Identification Association analysis Associated region annotation (with reference genome) -
Renewable Design for High Throughput Sequencing - Whole genome bisulfite sequencing – Biomarker
Service Advantages Sample Requirements Service Specifications Platform: NovaSeq 6000 platform, PE150 Recommended Sequencing Depth :≥30× coverage Service Work Flow Experiment design Sample delivery DNA extraction Library construction Sequencing Data analysis After-sale services -
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Fast delivery Dna And Rna Sequencing - Evolutionary Genetics – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Whole genome resequencing ü Data processing ü SNP/Indel calling ü Candidate region screening ü Candidate gene function annotation Sample Requirements and Delivery Sample Requirements: gDNA sample Tissue sample Concentration: ≥30 ng/μl Plants: 1-2 g Amount: ≥2 μg (Volumn ≥15 μl) Animals: 0.5-1 g Purity: OD260/280= 1.6-2.5 Whole blood: 1.5 ml Service Work Flow ... -
Factory wholesale Wgs Service - Amplicon (16S/18S/ITS) – Biomarker
Data Processing Raw data quality control Operational Taxonomic Unit(OTU) Clustering Taxonomy Species annotation Phylogenetic tree Components structure Alternative splicing Heatmap on species abundance Alpha Diversity Alpha-diversity metrics Rarefaction curve Shannon-Wiener curve Venn diagram Species accumulation curve Beta Diversity PCoA PCA UPGMA NMDS Advanced analysis Differential analysis LEfSe Intergroup correlation analysis Ternary phase diagram A...
