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Europe style for Intestinal Microbiome - Eukaryotic mRNA sequencing – Illumina – Biomarker
Advantages Sample Requirements and Delivery Library Sequencing strategy Data recommended Quality Control Poly A enriched Illumina PE150 6 Gb Q30≥85% Sample Requirements: Nucleotides: Purity Integrity Amount OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg Tissue: Weight(dry): ≥1 g *For t...
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factory customized Mrna Sequencing Provider - DNA sequencing -PacBio Sequencer – Biomarker
Features Two sequencing mode on PacBio sequencer: CLR and CCS. Mode Library Data yield Single-base accuracy Applications CLR 20Kb, 30Kb, etc. 80 Gb to 130 Gb/cell Approx. 85% De novo, SV calling, etc. CCS 15-20 Kb 14-40 Gb/cell (CCS) Approx. 99% De novo, Iso-Seq, SNP/Indel/SV calling Service Advantages Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Appearance Electrophoresis Gel Amount Others OD260/280=1.6-2.3; OD260/230=...
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2021 Latest Design V4 Region Of 16s Rrna - Nanopore Full-length transcriptomics – Biomarker
Bioinformatics Raw data quality control Full-length transcript assessment Transcript de-redundance Gene family Gene structure optimization Novel transcript prediction Alternative splicing Function annotation Fusion transcript identification lncRNA prediction SSR analysis CDS prediction ...
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Chinese Professional Metagenomics Data Analysis - Hi-C based Genome Assembly – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Raw data quality contro l ü Hi-C library quality control ü Hi-C based genome assembly ü Post-assembly evaluation Sample Requirements and Delivery Sample Requirements: For animals: 1.Muscle (Frozen): ≥ 2 g; 2.Whole blood (Non-frozen): ≥ 2 ml (for 3 libraries) For plants: 1.Seedlings (e.g. young seedlings cultivated from seeds, tissue culture seedingling, etc) : collect all leaves and young stem...
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Manufacturer for Structure Variation - circ-RNA – Biomarker
Bioinformatics Raw data quality control Reference genome alignment CircRNA identification circRNA locus circRNA conservation circRNA expression Novel circRNA prediction circRNA alternative splicing circRNA differential expression analysis circRNA-mRNA expression correlation Reproducibility test ...
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Factory Promotional Next Generation Sequencing Provider - mRNA(Reference) – Biomarker
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Europe style for Metagenomics And Microbiome - Nanopore Full-length transcriptomics – Biomarker
Bioinformatics Raw data quality control Full-length transcript assessment Transcript de-redundance Gene family Gene structure optimization Novel transcript prediction Alternative splicing Function annotation Fusion transcript identification lncRNA prediction SSR analysis CDS prediction ...
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Low price for Rna Sequencing Nanopore - Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq) – Biomarker
Service Advantages Service Specifications Sample Requirements Sample type: Tissues, live cells, or frozen cells and so on Cell number: ≥ 5×105 cells, an accurate cell number is key to success of the experiment; Tissue weight: ≥ 200mg fresh tissue; Service Work Flow Experiment design Sample delivery DNA extraction Library construction Sequencing Data analysis After-sale services
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New Fashion Design for Shotgun Sequencing Cost - DNA sequencing -PacBio Sequencer – Biomarker
Features Two sequencing mode on PacBio sequencer: CLR and CCS. Mode Library Data yield Single-base accuracy Applications CLR 20Kb, 30Kb, etc. 80 Gb to 130 Gb/cell Approx. 85% De novo, SV calling, etc. CCS 15-20 Kb 14-40 Gb/cell (CCS) Approx. 99% De novo, Iso-Seq, SNP/Indel/SV calling Service Advantages Sample Requirements and Delivery Sample Requirements: Nucleotides: Purity Appearance Electrophoresis Gel Amount Others OD260/280=1.6-2.3; OD260/230=...
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OEM Customized Mrna Seq Service - circ-RNA – Biomarker
Bioinformatics Raw data quality control Reference genome alignment CircRNA identification circRNA locus circRNA conservation circRNA expression Novel circRNA prediction circRNA alternative splicing circRNA differential expression analysis circRNA-mRNA expression correlation Reproducibility test ...
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Renewable Design for Illumina Rna Seq - Metabolomics – Biomarker
Sample Requirements Non-targeted metabolomics Sample types Recommended sample amount Biological Repetition LC-MS/GC-MS untargeted metabolomics Tissues 200mg Plant ≥6; Animal ≥10; Human ≥30; Plasma/serum 200ul Fecal/Intestinal Contents 150mg Cells 1*106 urine 500ul Rumen fluid 1ml Targeted metabolomics Sample types Recommended sample amount Biological Repetition Phytohormone/Tryptophane/Energy Metabolism/ Oxidized lipids/ Caroteno...
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Renewable Design for High Throughput Sequencing - Eukaryotic mRNA sequencing – Illumina – Biomarker
Advantages Sample Requirements and Delivery Library Sequencing strategy Data recommended Quality Control Poly A enriched Illumina PE150 6 Gb Q30≥85% Sample Requirements: Nucleotides: Purity Integrity Amount OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg Tissue: Weight(dry): ≥1 g *For t...
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Chinese Professional Briefings In Bioinformatics - Long non-coding sequencing – Illumina – Biomarker
Service Advantages Service Specifications Bioinformatics analyses ü Whole genome resequencing ü Data processing ü SNP/Indel calling ü Candidate region screening ü Candidate gene function annotation Sample Requirements and Delivery Sample Requirements: gDNA sample Tissue sample Concentration: ≥30 ng/μl Plants: 1-2 g Amount: ≥2 μg (Volumn ≥15 μl) Animals: 0.5-1 g Purity: OD260/280= 1.6-2.5 Whole blood: 1.5 ml Service Work Flow ...
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