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Factory wholesale Dna Sequencing Analysis - Nanopore-based metagenome sequencing – Biomarker

Service Details

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With the "Client-Oriented" business philosophy, a rigorous quality control system, advanced manufacturing equipment and a strong R&D team, we always provide high quality products, excellent services and competitive prices for Transcriptional Profiling , Bgi Sequencing , Genome Wide Association Studies Provider, We have now a experienced crew for international trade. We are able to solve the problem you meet. We are able to offer the products and solutions you want. You should really feel free of charge to speak to us.

Factory wholesale Dna Sequencing Analysis - Nanopore-based metagenome sequencing – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data quality control

ü Metagenome assembly

ü Non-redundant gene set and annotation

ü Species diversity analysis

ü Genetic function diversity analysis

ü Inter-group analysis

ü Association analysis against experimental factors

Sample Requirements and Delivery

Sample requirements and delivery

Sample Requirements:

For DNA extracts:

Sample Type	Amount	Concentration	Purity
DNA extracts	＞ 30 ng	＞ 1 ng/μl	OD260/280= 1.6-2.5

For environmental samples:

Sample type	Recommended sampling procedure
Soil	Sampling amount: approx. 5 g; Remaining withered substance needs to be removed from surface; Grind large pieces and pass through 2 mm filter; Aliquot samples in sterile EP-tube or cyrotube for reservation.
Feces	Sampling amount: approx. 5 g; Collect and aliquot samples in sterile EP-tube or cryotube for reservation.
Intestinal contents	Samples need to be processed under aseptic condition. Wash collected tissue with PBS; Centrifuge the PBS and collect the precipitant in EP-tubes.
Sludge	Sampling amount: approx. 5 g; Collect and aliquot sludge sample in sterile EP-tube or cryotube for reservation
Waterbody	For sample with limited amount of microbial, such as tap water, well water, etc., Collect at least 1 L water and pass through 0.22 μm filter to enrich microbial on the membrane. Store the membrane in sterile tube.
Skin	Carefully scrape skin surface with sterile cotton swab or surgical blade and place it in sterile tube.

Recommended Sample Delivery

Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

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