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Factory Supply Rna Sequencing Workflow - Nanopore-based metagenome sequencing – Biomarker

Service Details

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Factory Supply Rna Sequencing Workflow - Nanopore-based metagenome sequencing – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data quality control

ü Metagenome assembly

ü Non-redundant gene set and annotation

ü Species diversity analysis

ü Genetic function diversity analysis

ü Inter-group analysis

ü Association analysis against experimental factors

Sample Requirements and Delivery

Sample requirements and delivery

Sample Requirements:

For DNA extracts:

Sample Type	Amount	Concentration	Purity
DNA extracts	＞ 30 ng	＞ 1 ng/μl	OD260/280= 1.6-2.5

For environmental samples:

Sample type	Recommended sampling procedure
Soil	Sampling amount: approx. 5 g; Remaining withered substance needs to be removed from surface; Grind large pieces and pass through 2 mm filter; Aliquot samples in sterile EP-tube or cyrotube for reservation.
Feces	Sampling amount: approx. 5 g; Collect and aliquot samples in sterile EP-tube or cryotube for reservation.
Intestinal contents	Samples need to be processed under aseptic condition. Wash collected tissue with PBS; Centrifuge the PBS and collect the precipitant in EP-tubes.
Sludge	Sampling amount: approx. 5 g; Collect and aliquot sludge sample in sterile EP-tube or cryotube for reservation
Waterbody	For sample with limited amount of microbial, such as tap water, well water, etc., Collect at least 1 L water and pass through 0.22 μm filter to enrich microbial on the membrane. Store the membrane in sterile tube.
Skin	Carefully scrape skin surface with sterile cotton swab or surgical blade and place it in sterile tube.

Recommended Sample Delivery

Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

Product detail pictures:

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"Based on domestic market and expand overseas business" is our improvement strategy for Factory Supply Rna Sequencing Workflow - Nanopore-based metagenome sequencing – Biomarker , The product will supply to all over the world, such as: Bangalore , Stuttgart , Armenia , Our professional engineering group will always be ready to serve you for consultation and feedback. We're able to also offer you with absolutely free samples to meet your requirements. Finest efforts will likely be produced to offer you the ideal service and goods. For anyone who is thinking about our company and merchandise, be sure to contact us by sending us emails or contact us quickly. As a way to know our merchandise and firm. lot more, you can come to our factory to find out it. We'll always welcome guests from all over the world to our business to build company relations with us. Be sure to feel free to get in touch with us for business and we believe we've been intending to share the top trading practical experience with all our merchants.

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