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Factory making Strand-Specific Rna-Seq - Full-length mRNA sequencing -Nanopore – Biomarker

Service Details

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We know that we only thrive if we can guarantee our combined rate competiveness and good quality advantageous at the same time for Pacbio Sequel Ii Service , Rna Sequencing Cost , Hi C Data Analysis , We have been one of your largest 100% manufacturers in China. A lot of large trading businesses import products and solutions from us, so we can easily give you the most beneficial price tag with the same quality for anyone who is interested in us.

Factory making Strand-Specific Rna-Seq - Full-length mRNA sequencing -Nanopore – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data processing

ü Transcript identification

ü Alternative splicing

ü Expression quantification in gene level and isoform level

ü Differential expression analysis

ü Function annotation and enrichment (DEGs and DETs)

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Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel.	For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥40 ng/μl;Total ≥ 1 μg

Tissue: Weight(dry): ≥1 g

*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷

*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×10⁵, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.

RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

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Bioinformatics Company

Genomics And Metagenomics

Rna Seq Technology

Selective Sweep

Transcriptome

Transcriptome Analysis Methods

The factory can meet continuously developing economic and market needs, so that their products are widely recognized and trusted, and that's why we chose this company.