"Based on domestic market and expand overseas business" is our development strategy for
16s Bacteria
, Rna Seq Technology
, Eukaryotic Mrna
, With superb service and quality, and an enterprise of foreign trade featuring validity and competitiveness, that will be trusted and welcomed by its clients and creates happiness to its employees.
Factory making Strand-Specific Rna-Seq -
Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:
Advantages
Sample Requirements and Delivery
Library |
Sequencing strategy |
Data recommended |
Quality Control |
Poly A enriched |
Illumina PE150 |
6 Gb
|
Q30≥85% |
Sample Requirements:
Nucleotides:
Purity |
Integrity |
Amount |
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation |
Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
- Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
That has a positive and progressive attitude to customer's interest, our organization consistently improves our products quality to satisfy the demands of shoppers and further focuses on safety, reliability, environmental specifications, and innovation of
Factory making Strand-Specific Rna-Seq -
Eukaryotic mRNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: European
, Poland
, Germany
, Our tenet is "integrity first, quality best". We have confidence in providing you with excellent service and ideal products. We sincerely hope we can establish win-win business cooperation with you in the future!