factory customized Mrna Sequencing Provider - Full-length mRNA sequencing-Nanopore – Biomarker
factory customized Mrna Sequencing Provider - Full-length mRNA sequencing-Nanopore – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data processing
ü Transcript identification
ü Alternative splicing
ü Expression quantification in gene level and isoform level
ü Differential expression analysis
ü Function annotation and enrichment (DEGs and DETs)
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Purity | Integrity | Amount |
| OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel. | For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation | Conc. ≥40 ng/μl;Total ≥ 1 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
Being supported by an innovative and experienced IT team, we could present technical support on pre-sales & after-sales service for factory customized Mrna Sequencing Provider - Full-length mRNA sequencing-Nanopore – Biomarker , The product will supply to all over the world, such as: Oslo , Miami , Thailand , Based on products with high quality, competitive price, and our full range service, we have accumulated professional strength and experience, and we have built up a very good reputation in the field. Along with the continuous development, we commit ourselves not only to the Chinese domestic business but also the international market. May you moved by our high quality products and passionate service. Let's open a new chapter of mutual benefit and double win.
By Louise
from Eindhoven
- 2017.01.28 19:59
Although we are a small company, we are also respected. Reliable quality, sincere service and good credit, we are honored to be able to work with you!
By Tyler Larson
from Iceland
- 2018.05.15 10:52
