Products

Factory Cheap Bioinformatics Analysis - Eukaryotic mRNA sequencing – Illumina – Biomarker

Service Details

Feedback (2)

We will make every effort to be outstanding and perfect, and accelerate our steps for standing in the rank of international top-grade and high-tech enterprises for Oxford Nanopore , Plant Genome Sequencing , Contig Assembly , Our merchandise are widely recognized and trusted by users and can meet up with continuously changing economic and social needs.

Factory Cheap Bioinformatics Analysis - Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:

Advantages

Sample Requirements and Delivery

Library	Sequencing strategy	Data recommended	Quality Control
Poly A enriched	Illumina PE150	6 Gb	Q30≥85%

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel.	For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Microorganism: Mass ≥ 1 g

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:

Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

logo_01

Experiment design

logo_02

Sample delivery

logo_03

RNA extraction

logo_04

Library construction

logo_05

Sequencing

logo_06

Data analysis

logo_07

After-sale services

Product detail pictures:

Related Product Guide:

We not only will try our greatest to present fantastic expert services to each purchaser, but also are ready to receive any suggestion offered by our prospects for Factory Cheap Bioinformatics Analysis - Eukaryotic mRNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Stuttgart , Cyprus , Palestine , Today, We are with great passion and sincerity to further fulfill our global customers' needs with good quality and design innovation. We fully welcome customers from all over the world to establish stable and mutually beneficial business relationships, to have a bright future together.

Cdna Library