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Excellent quality Basic Bioinformatics -
circRNA sequencing – Illumina – Biomarker Detail:
Service Advantages
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
Purity |
Integrity |
Contamination |
Amount |
OD260/280≥1.7-2.5;OD260/230≥0.5-2.5; |
For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation |
Limited or no protein or DNA contamination shown on gel. |
Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended.
Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
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Excellent quality Basic Bioinformatics -
circRNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Sao Paulo
, Ukraine
, Montpellier
, Taking the core concept of "to be the Responsible". We'll redound up on society for high quality merchandise and good service. We will initiative to participate in international competition to be a first- class manufacturer of this product in the world.