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DNA sequencing -PacBio Sequencer – Biomarker

Service Details

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DNA sequencing -PacBio Sequencer – Biomarker Detail:

Features

Two sequencing modes on PacBio sequencer: CLR and CCS.

Mode	Library	Data yield	Single-base accuracy	Applications
CLR	20Kb, 30Kb, etc.	80 Gb to 130 Gb/cell	Approx. 85%	De novo, SV calling, etc.
CCS	15-20 Kb	14-40 Gb/cell (CCS)	Approx. 99%	De novo, Iso-Seq, SNP/Indel/SV calling

Service Advantages

Sample Requirements

Nucleotides:

Purity	Appearance	Electrophoresis Gel	Amount	Others
OD260/280=1.6-2.3; OD260/230=1.5-2.5； Clear peak at 260 nm	Colourless, transparent liquid.	Clear main band, limited degradation observed, no or limited protein, poly-saccharides or RNA contaminations	Conc. ≥20 ng/μl; Volumn ≥15μl; Total amount depends on requirements in data yield.	Concentration needs to be measured by Qubit and Qubit/Nanopore = 0.8-2.0

*NC/QC: NanoDrop concentration/Qubit concentration

Service Work Flow

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Sample preparation

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Library construction

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Sequencing

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Data analysis

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Project delivery

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