● Advanced Bioinformatic Analysis and Comprehensive Annotation: We use multiple databases to functionally annotate the genes associated with regions of protein-DNA binding, providing insights on the cellular and molecular processes underlying the interaction.
● Post-Sales Support: Our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
● Extensive Experience: With a track record of successfully completing numerous ChIP-Seq projects, our company brings over a decade of expertise to the table. Our highly skilled analysis team, coupled with comprehensive content and post-sales support, ensures the success of your projects.
● Rigorous Quality Control: We implement core control points across all stages, from sample and library preparation to sequencing and bioinformatics. This meticulous monitoring ensures the delivery of consistently high-quality results.
Library |
Sequencing Strategy |
Recommended data output |
Quality control |
Purified DNA after Immunoprecipitation |
Illumina PE150 |
10Gb |
Q30≥85% Bisulfite conversion >99% MspI cutting efficiency > 95% |
Total amount: ≥10 ng
Fragment size distribution: 100-750 bps
Includes the following analysis:
● Raw data quality control
● Peak calling based on mapping to reference genome
● Annotation of peak-associated genes
● Motif analysis: identification of transcription factor binding sites (TFBS)
● Differential Peak Analysis and annotation
Evaluation of enrichment near Transcription Starting Sites (TSSs)
Genome-wide distribution of CHIP peaks
Classification of peak regions
Functional enrichment of peak-related genes (KEGG)