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China Gold Supplier for Variation Detection - Full-length mRNA sequencing -Nanopore – Biomarker

Service Details

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Our commission should be to provide our customers and consumers with ideal top quality and aggressive portable digital products for Ngs Cost , Oxford Nanopore Pricing , Dna Sequencing , We sincerely hope to serve you and your business with a good start. If there is anything we can do for You, we shall be more than pleased to do so. Welcome to our factory for visit.

China Gold Supplier for Variation Detection - Full-length mRNA sequencing -Nanopore – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data processing

ü Transcript identification

ü Alternative splicing

ü Expression quantification in gene level and isoform level

ü Differential expression analysis

ü Function annotation and enrichment (DEGs and DETs)

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Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel.	For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥40 ng/μl;Total ≥ 1 μg

Tissue: Weight(dry): ≥1 g

*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷

*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×10⁵, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.

RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

Product detail pictures:

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A nice supplier in this industry, after a detail and careful discussion, we reached a consensus agreement. Hope that we cooperate smoothly.

By Lisa from Hanover - 2017.06.29 18:55

This is a reputable company, they have a high level of business management, good quality product and service, every cooperation is assured and delighted!

By Queena from Belgium - 2018.11.06 10:04