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Big discounting De Novo Genome Assembly - De novo Full-length Transcriptome sequencing -PacBio – Biomarker

Service Details

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We've our have sales staff, style and design staff, technical crew, QC team and package workforce. We've strict excellent control procedures for each system. Also, all of our workers are experienced in printing field for Pacbio Sequel Ii , Gene Flow , Micro Rna Sequencing , Adhering to the business enterprise philosophy of 'customer first, forge ahead', we sincerely welcome consumers from at your home and abroad to cooperate with us provide you greatest services!

Big discounting De Novo Genome Assembly - De novo Full-length Transcriptome sequencing -PacBio – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data processing

ü Transcript identification

ü Sequence structure

ü Expression Quantification

ü Function Annotation

Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Amount	Total ≥ 3 μg; Conc. ≥ 300 ng/μl; Volumn ≥ 10 μl
Integrity	For plants: RIN ≥ 7.5; For animals: RIN ≥ 8; 28S/18S ≥ 1.0; Limited or no baseline elevation
Purity	OD260/280 ≥ 1.8; OD260/230 ≥ 1.0; Clear peak at 260 nm
Note	Protein or DNA contamination is very likely to affect the measurements and the samples’ QC level.

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ – 1×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Microorganism: Mass ≥ 1 g

Recommended Sample Delivery

Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:

1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

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